Expression of COX-1, COX-2, and PPAR-gamma in the gastric mucosa of children with Helicobacter pylori infection.

Abstract

BACKGROUND:

Gastric inflammation in patients with Helicobacter pylori infection is considered to be regulated by many kinds of inflammatory and cytoprotective factors. The present study examined the effects of cyclo-oxygenase (COX)-1, -2, and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) on gastric mucosal injury in children with H. pylori infection.

METHODS:

The subjects were 24 children who underwent endoscopy for the evaluation of anemia or gastrointestinal symptoms, and they were divided into two groups: a H. pylori-positive group and -negative group. The numbers of neutrophils in the gastric mucosa of children with and without H. pylori infection and expression of COX-1, -2, and PPAR-gamma were examined, using reverse transcription-polymerase chain reaction.

RESULTS:

The numbers of neutrophils were significantly higher in the H. pylori-positive group than in the H. pylori-negative group. The ratio of COX-1 mRNA to COX-2 mRNA in the H. pylori-positive group was significantly lower than that in the H. pylori-negative group. The ratio of PPAR-gamma m-RNA to beta-actin mRNA was significantly higher in the H. pylori-positive group than the H. pylori-negative group.

CONCLUSIONS:

Enhanced production of COX-2 and PPAR-gamma in the gastric mucosa has cytoprotective and anti-inflammatory effects, although the relationship to the carcinogenic activity of COX-2 and PPAR-gamma should be clarified.

• Authors: Hidenori Haruna, Toshiaki Shimizu, Yoshikazu Ohtsuka, Yukiko Yarita, Tohru Fujii, Takahiro Kudo, Yuichiro Yamashiro
• Journal: Pediatrics international : official journal of the Japan Pediatric Society (Pediatr Int) Vol. 50 Issue 1 Pg. 1-6 (Feb 2008) ISSN: 1328-8067 [Print] Australia
• PMID: 18279196 (Publication Type: Journal Article)

Chemical References

• PPAR gamma
• RNA, Messenger
• Cyclooxygenase 1
• Cyclooxygenase 2

Topics

• Adolescent
• Child
• Child, Preschool
• Cyclooxygenase 1 (analysis)
• Cyclooxygenase 2 (analysis)
• Female
• Gastric Mucosa (chemistry)
• Helicobacter Infections (metabolism)
• Helicobacter pylori
• Humans
• Infant
• Male
• PPAR gamma (analysis)
• RNA, Messenger (analysis)