Identification of specific protein markers in microdissected hepatocellular carcinoma.
|
| Abstract |
At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers.
|
|---|---|
| Authors | Christian Melle, Günther Ernst, Olaf Scheibner, Roland Kaufmann, Bettina Schimmel, Annett Bleul, Utz Settmacher, Merten Hommann, Uwe Claussen, Ferdinand von Eggeling |
| Journal | Journal of proteome research (J Proteome Res) Vol. 6 Issue 1 Pg. 306-15 (Jan 2007) ISSN: 1535-3893 [Print] United States |
| PMID | 17203974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!