Role of intercellular adhesion molecule-1 in a murine model of toluene diisocyanate-induced asthma.

Abstract

BACKGROUND:

Adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) are thought to contribute to the airway inflammation and airway hyper-responsiveness (AHR) of allergic asthma. Some differences from allergic asthma have been noted, including airway neutrophilia, and the involvement of ICAM-1 in toluene diisocyanate (TDI) asthma is currently unclear.

OBJECTIVE:

We utilized mice lacking ICAM-1 expression (ICAM-1(-/-)) to investigate the role of ICAM-1 in airway inflammation and AHR in TDI-induced asthma.

METHODS:

Male C57BL/6J mice (ICAM-1(+/+)) and ICAM-1(-/-) mice were intranasally sensitized to TDI solution or solvent alone. Airway inflammation, AHR and cytokine secretion were assessed 24 h after challenge by TDI or solvent. The production of antigen-specific IgG and IgE by TDI sensitized and non-sensitized mice was determined.

RESULTS:

TDI challenge to ICAM-1(+/+) mice induced an increase in airway inflammatory cell numbers, AHR and cytokine secretion of TNF-alpha, macrophage inflammatory protein-2 (MIP-2), IL-4, IL-5 and IFN-gamma into the bronchoalveolar lavage fluid. All these pathophysiological changes were reduced in ICAM-1(-/-) mice. Serum levels of TDI-specific IgG and IgE of ICAM-1(-/-) and ICAM-1(+/+) mice were comparable.

CONCLUSION:

These results suggest that ICAM-1 plays an essential role in airway inflammation and AHR in TDI-induced asthma.

• Authors: S Furusho, S Myou, M Fujimura, T Kita, M Yasui, K Kasahara, S Nakao, K Takehara, S Sato
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 36 Issue 10 Pg. 1294-302 (Oct 2006) ISSN: 0954-7894 [Print] England
• PMID: 17014439 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bronchoconstrictor Agents
• Chemokine CXCL2
• Chemokines
• Cxcl2 protein, mouse
• Immunoglobulin G
• Interleukin-5
• Tumor Necrosis Factor-alpha
• Methacholine Chloride
• Intercellular Adhesion Molecule-1
• Toluene 2,4-Diisocyanate
• Interleukin-4
• Immunoglobulin E
• Interferon-gamma

Topics

• Animals
• Asthma (chemically induced, immunology, metabolism)
• Bronchial Hyperreactivity
• Bronchial Provocation Tests
• Bronchoalveolar Lavage Fluid (immunology)
• Bronchoconstrictor Agents
• Chemokine CXCL2
• Chemokines (immunology)
• Immunoglobulin E (blood)
• Immunoglobulin G (blood)
• Immunohistochemistry (methods)
• Intercellular Adhesion Molecule-1 (analysis, genetics, physiology)
• Interferon-gamma (immunology)
• Interleukin-4 (immunology)
• Interleukin-5 (immunology)
• Lung (chemistry, immunology, metabolism)
• Male
• Methacholine Chloride
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Models, Animal
• Occupational Diseases (etiology, immunology, metabolism)
• Time Factors
• Toluene 2,4-Diisocyanate (adverse effects)
• Tumor Necrosis Factor-alpha (immunology)