Abstract | BACKGROUND: METHODS: RESULTS: Pulmonary hemorrhage appeared in the model guinea pig 24 hours after leptospires intraperitoneal inoculation, progressing to a peak at 96 hours after the infection. Leptospires were detected 24 hours post-inoculation in the liver, 48 hours in the lung and 72 hours in the kidney by immunohistochemistry staining. Spiral form of the bacteria was initially observed in the liver, lung and kidney suggestive of intact leptospires, granular form of leptospires was seen as the severity increased. Platelet aggregation in hepatic sinusoid as well as phagocytosis of erythrocytes and platelets by Kupffer cells were both observed. Neither platelet thrombus nor fibrin thrombus was found in the liver, lung or kidney via morphological observation. Thrombocytopenia was observed in all infected guinea pigs of our experimental leptospirosis study. Analysis of hematologic molecular markers showed that 11-DH-TXB2 and TM in the plasma were elevated significantly. TAT that reflects the thrombin activation had a trend of decline after infection. Although D-dimer and FDPs increased statistically, the increasing may not bear clinical significance. CONCLUSION: Pathologic and hematological studies for experimental leptospirosis of guinea pig indicated that the thrombocytopenia found in guinea pigs did not correlate with the occurrence of DIC. The platelet aggregation and Kupffer cells phagocytosis might be the potential causes of thrombocytopenia in severe leptospirosis.
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Authors | Hong-Liang Yang, Xu-Cheng Jiang, Xiang-Yan Zhang, Wen-Jun Li, Bao-Yu Hu, Guo-Ping Zhao, Xiao-Kui Guo |
Journal | BMC infectious diseases
(BMC Infect Dis)
Vol. 6
Pg. 19
(Feb 02 2006)
ISSN: 1471-2334 [Electronic] England |
PMID | 16451735
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Disease Models, Animal
- Disseminated Intravascular Coagulation
(etiology, microbiology, pathology)
- Erythrocyte Deformability
- Female
- Guinea Pigs
- Hemorrhage
(etiology, pathology)
- Immunohistochemistry
(methods)
- Kidney
(pathology)
- Leptospira interrogans
- Leptospirosis
(complications, diagnosis, microbiology, pathology)
- Liver
(pathology, ultrastructure)
- Lung
(pathology)
- Lung Diseases
(etiology, pathology)
- Male
- Platelet Aggregation
- Platelet Count
- Thrombocytopenia
(etiology, pathology)
- Time Factors
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