Different roles of interleukin-10 in onset and resolution of asthmatic responses in allergen-challenged mice.

Abstract

OBJECTIVE:

Although interleukin (IL)-10 is an immunoregulatory cytokine produced by various cells including T cells, its precise role in asthma remains uncertain. The aim of this study was to investigate the role of IL-10 in experimental asthma using ovalbumin (OVA)-sensitized mice.

METHODOLOGY:

Mice were challenged with OVA aerosol, and airway responsiveness and inflammation were measured. OVA-specific IL-10-producing CD4+ T cells were counted from lung cells collected by enzymatic digestion and stimulated ex vivo with OVA. The effects of an anti-IL-10 antibody on airway responsiveness and inflammation were also evaluated.

RESULTS:

The OVA challenge caused airway hyperresponsiveness and eosinophilic inflammation. A significant increase in IL-10-producing CD4+ T cells was observed, mainly in the CD45RB(low) subset, for several days after the OVA challenge. Anti-IL-10 antibody treatment before the OVA challenge did not affect eosinophilic inflammation but significantly inhibited airway hyperresponsiveness 24 h after the OVA challenge. However, anti-IL-10 antibody treatment just before the last OVA challenge significantly attenuated the resolution of eosinophilic inflammation without affecting airway responsiveness 2 weeks after the OVA challenge.

CONCLUSIONS:

Intrinsic IL-10 may have a distinct role in the early and late phases of asthmatic responses. In the early phase, IL-10 induces airway hyperresponsiveness, while in the late phase IL-10 contributes to the resolution of eosinophilic inflammation.

• Authors: Koichiro Matsumoto, Hiromasa Inoue, Miyuki Tsuda, Yasuko Honda, Atsuko Kibe, Kentaro Machida, Yuki Yoshiura, Yoichi Nakanishi
• Journal: Respirology (Carlton, Vic.) (Respirology) Vol. 10 Issue 1 Pg. 18-26 (Jan 2005) ISSN: 1323-7799 [Print] Australia
• PMID: 15691233 (Publication Type: Journal Article)

Chemical References

• Allergens
• Antibodies
• Interleukin-5
• Interleukin-10
• Ovalbumin

Topics

• Allergens (adverse effects)
• Animals
• Antibodies (immunology)
• Asthma (immunology, physiopathology)
• Bronchi (immunology)
• Bronchial Hyperreactivity (immunology)
• Bronchoalveolar Lavage Fluid (immunology)
• CD4 Lymphocyte Count
• CD4-Positive T-Lymphocytes (immunology)
• Eosinophils (immunology)
• Immunization
• Interleukin-10 (immunology)
• Interleukin-5 (immunology)
• Male
• Mice
• Mice, Inbred BALB C
• Ovalbumin (adverse effects)
• T-Lymphocyte Subsets (immunology)
• Time Factors