Abstract | OBJECTIVES: METHODS: In a randomized, blinded study with a porcine left single- lung transplantation model, donor lungs were flushed with 1 L of either low- potassium dextran solution or low- potassium dextran solution modified by the addition of 250 microg iloprost (n = 6 in each group). Grafts were stored at 4 degrees C for 24 hours. After transplantation, the right bronchus and pulmonary artery were clamped, and the animals remained dependent on the graft. Posttransplantation graft function was assessed throughout a 7-hour observation period by measuring oxygenation (30-minute intervals), different pulmonary and systemic hemodynamic parameters, and wet/dry lung weight ratios. Bronchoalveolar lavage fluid was obtained before and 2 hours after reperfusion. Surfactant function was measured from bronchoalveolar lavage fluid with a pulsating bubble surfactometer. Neutrophil sequestration was assessed by a myeloperoxidase assay performed on lung tissue specimens taken at the end of the observation period. RESULTS: Pulmonary vascular resistance remained lower in the iloprost group than in the control group (P < .05). Tissue water content after 7 hours of reperfusion remained lower in the iloprost group (P < .05). In addition, significantly reduced myeloperoxidase tissue activity was observed in the iloprost group (P < .05). Although there was no difference in degradation of surface active surfactant large aggregates to small aggregates, the surface tension measured at minimal bubble diameter was lower in the iloprost group (P < .05). CONCLUSIONS:
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Authors | Bernhard Gohrbandt, Sebastian P Sommer, Stefan Fischer, Jens M Hohlfeld, Gregor Warnecke, Axel Haverich, Martin Strueber |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 129
Issue 1
Pg. 80-6
(Jan 2005)
ISSN: 0022-5223 [Print] United States |
PMID | 15632828
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Organ Preservation Solutions
- Pulmonary Surfactants
- Peroxidase
- Iloprost
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Topics |
- Animals
- Disease Models, Animal
- Female
- Graft Rejection
(prevention & control)
- Graft Survival
- Iloprost
(pharmacology)
- Lung Transplantation
(adverse effects, methods)
- Male
- Organ Preservation
(methods)
- Organ Preservation Solutions
(pharmacology)
- Peroxidase
(metabolism)
- Probability
- Pulmonary Surfactants
- Random Allocation
- Reference Values
- Reperfusion Injury
(prevention & control)
- Sensitivity and Specificity
- Surface Tension
(drug effects)
- Swine
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