Cloning of IP15, a pancreatitis-induced gene whose expression inhibits cell growth.

Abstract

We describe the cloning and expression of the mouse gene interferon-inducible-protein 15 (IP15), whose activation is related to the acute phase of experimental pancreatitis. Analysis of its structure indicates that it encodes a putative transmembrane protein of 137 amino acids. This gene contains a predicted IFN-stimulable-response element. In vivo studies showed that IP15 is strongly activated in pancreas early during caerulein-induced pancreatitis. In situ hybridization of IP15 mRNA showed that its expression is restricted to acinar cells. IP15 was also induced in pancreas under systemic-lipopolysaccharide treatment and in intestine under Salmonella infection. In vitro studies using NIH3T3 fibroblasts showed that IP15 is induced by IFN-alpha. Growth rate was significantly lower in cells transfected with pcDNA4/IP15 plasmid. In addition, cells expressing IP15 showed less capacity to develop colonies after antibiotic selection. In conclusion, we identified a new interferon-inducible gene that is activated early in pancreas with pancreatitis and whose expression inhibits cell growth.

• Authors: Alejandro Ropolo, Richard Tomasini, Daniel Grasso, Nelson J Dusetti, María C Cerquetti, Juan L Iovanna, María I Vaccaro
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 319 Issue 3 Pg. 1001-9 (Jul 02 2004) ISSN: 0006-291X [Print] United States
• PMID: 15184081 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IP15 protein, mouse
• Membrane Proteins
• RNA, Messenger
• Ceruletide
• Interferons

Topics

• Amino Acid Sequence
• Animals
• Cell Division (physiology)
• Cell Line
• Ceruletide (toxicity)
• Cloning, Molecular
• Female
• Fetus (physiology)
• Humans
• In Situ Hybridization
• Interferons (metabolism)
• Membrane Proteins (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Pancreas (cytology, physiology)
• Pancreatitis (chemically induced, genetics, metabolism, pathology)
• Pregnancy
• RNA, Messenger (metabolism)
• Response Elements
• Sequence Alignment
• Sequence Homology, Amino Acid