Up-regulation of C5a receptor expression and function on human monocyte derived dendritic cells by prostaglandin E2.

Abstract

The expression of the C5a-receptor (C5aR) on dendritic cells, its regulation and function have not been well established thus far. We show that the C5aR is expressed on human monocyte-derived dendritic cells (DC) and can be down-regulated by maturation stimuli such as tumour necrosis factor-alpha (TNF-alpha), lipopolysaccharide (LPS) or CD40L and by the T helper 1-cytokine interferon-gamma (INF-gamma). Prostaglandin E2 (PGE2), a proinflammatory mediator supporting dendritic cell activation and necessary for adequate DC migration, leads to the up-regulation of C5aR expression when incubated alone and prevents down-regulation when given in combination with TNF-alpha or LPS. Stimulation of C5aR on DC triggered F-actin polymerization, indicating the chemotactic potential of DC elicited by C5a. C5a induced F-actin polymerization was increased when C5aR was up-regulated by PGE2. Stimulation of DC with C5a resulted in interleukin-10 production which was significantly increased after C5aR up-regulation with TNF-alpha and PGE2. Therefore, up-regulation of the C5aR on human DC alters their chemotactic and immunologic response to C5a.

• Authors: Oliver Weinmann, Ralf Gutzmer, Jörg Zwirner, Miriam Wittmann, Katja Langer, Margarete Lisewski, Susanne Mommert, Alexander Kapp, Thomas Werfel
• Journal: Immunology (Immunology) Vol. 110 Issue 4 Pg. 458-65 (Dec 2003) ISSN: 0019-2805 [Print] England
• PMID: 14632643 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Actins
• CD40 Antigens
• Lipopolysaccharides
• Polymers
• Receptor, Anaphylatoxin C5a
• Tumor Necrosis Factor-alpha
• Interleukin-10
• Interferon-gamma
• Dinoprostone

Topics

• Actins (immunology, metabolism)
• CD40 Antigens (immunology)
• Dendritic Cells (immunology)
• Dinoprostone (immunology)
• Down-Regulation (immunology)
• Flow Cytometry (methods)
• Humans
• Interferon-gamma (immunology)
• Interleukin-10 (biosynthesis, immunology)
• Lipopolysaccharides (immunology)
• Monocytes (immunology)
• Polymerase Chain Reaction (methods)
• Polymers (metabolism)
• Receptor, Anaphylatoxin C5a (immunology)
• Tumor Necrosis Factor-alpha (immunology)
• Up-Regulation (immunology)