Identification of MG-160, a FGF binding medial Golgi sialoglycoprotein, in brain tumors: an index of malignancy in astrocytomas.

Abstract

Malignant astrocytomas are highly invasive, vascular neoplasms that comprise the majority of nervous system tumors in humans. A strong association has previously been made between malignancy in human astrocytic tumors and increased expression of certain fibroblast growth factor (FGF) family members. MG-160 is an intrinsic type I cysteine-rich membrane sialoglycoprotein that resides in the medial cisternae of the Golgi apparatus, highly homologous to CFR, chicken fibroblast growth factor receptor, and ESL-1, E-selectin ligand. MG-160 binds fibroblast growth factors (FGFs) and may be involved in the intracellular trafficking of FGFs and the regulation of cellular response to FGFs. In the present study MG-160 expression was evaluated in human brain tumors exhibiting varying degrees of malignancy. At the mRNA level MG-160 expression was inversely correlated with the histological grade of astrocytomas such that high levels of MG-160 were observed in low-grade astrocytomas and low levels in malignant astrocytomas. This differential expression of MG-160 mRNA was verified at the protein level using immunohistochemistry. Grade II astrocytomas displayed consistent and intense staining for MG-160 as seen in normal brain. In contrast to the lower grade tumors, grade IV astrocytomas exhibited variable and weaker expression of MG-160. Our results suggest that, MG-160 may participate in malignant progression in astrocytomas. In addition, other brain tumors and human astrocytoma cell lines were characterized for MG-160 expression.

• Authors: Fumio Yamaguchi, Richard S Morrison, Nicholas K Gonatas, Hiroshi Takahashi, Yuichi Sugisaki, Akira Teramoto
• Journal: International journal of oncology (Int J Oncol) Vol. 22 Issue 5 Pg. 1045-9 (May 2003) ISSN: 1019-6439 [Print] Greece
• PMID: 12684670 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA Primers
• RNA, Messenger

Topics

• Astrocytoma (genetics, pathology)
• Base Sequence
• Brain Neoplasms (genetics, pathology)
• DNA Primers
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, pathology)
• Golgi Apparatus (genetics)
• Humans
• Immunohistochemistry
• Neoplasm Staging
• RNA, Messenger (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription, Genetic
• Tumor Cells, Cultured