Abstract | PURPOSE: METHODS: A rat carotid endarterectomy model was used in which an open technique with arteriotomy and intimectomy was used. Saratin was applied to the endarterectomized surface of the carotid artery before arterial closure. End point measurements included platelet adhesion, thrombosis rate, intimal hyperplasia development, bleeding times, and platelet counts. Electron micrographs of carotid arteries were used for quantitative analysis of platelet aggregation and platelet counts. Intimal hyperplasia and thrombosis were assessed with computer-assisted morphometric analysis of elastin-stained carotid artery sections with direct measurement of the intimal hyperplasia area. RESULTS: The topical application of saratin significantly decreased platelet adhesion compared with controls at 3 hours after carotid endarterectomy (64 +/- 17 vs 155 +/- 33 platelets per grid, P = .05), and 24 hours after carotid endarterectomy (35 +/- 11 vs 149 +/- 37 platelets per grid, P = .0110), respectively. A percent luminal stenosis, as a measure of intimal hyperplasia, was significantly decreased with saratin application compared with controls (10.9% +/- 1.8% vs 29.8% +/- 6.8%, P = .0042). This decrease in intimal hyperplasia formation correlated with the inhibition of platelet adhesion. Thirty-three percent of control arteries were found to be thrombosed 2 weeks after carotid endarterectomy compared with a 0% thrombosis rate in the saratin-treated group (P = .0156). No increased bleeding was encountered along the arterial suture line in the saratin group. Bleeding times and systemic platelet counts were not found to change significantly in the saratin-treated rats compared with control rats at 3 and 24 hours after endarterectomy. CONCLUSION:
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Authors | C P Cruz, J Eidt, J Drouilhet, A T Brown, Y Wang, C S Barnes, M M Moursi |
Journal | Journal of vascular surgery
(J Vasc Surg)
Vol. 34
Issue 4
Pg. 724-9
(Oct 2001)
ISSN: 0741-5214 [Print] United States |
PMID | 11668330
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Platelet Aggregation Inhibitors
- Salivary Proteins and Peptides
- saratin
- von Willebrand Factor
- Collagen
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Topics |
- Administration, Topical
- Animals
- Bleeding Time
- Carotid Arteries
(drug effects, ultrastructure)
- Carotid Stenosis
(etiology, prevention & control, surgery)
- Collagen
(drug effects, physiology)
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Endarterectomy, Carotid
(adverse effects)
- Hyperplasia
- Platelet Adhesiveness
(drug effects)
- Platelet Aggregation Inhibitors
(pharmacology, therapeutic use)
- Platelet Count
- Rats
- Rats, Sprague-Dawley
- Recurrence
- Salivary Proteins and Peptides
(pharmacology, therapeutic use)
- Stroke
(epidemiology, etiology, prevention & control)
- Thrombosis
(etiology, metabolism, prevention & control)
- Time Factors
- Tunica Intima
(drug effects, injuries, pathology, ultrastructure)
- von Willebrand Factor
(antagonists & inhibitors)
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