Abstract | BACKGROUND & AIMS: METHODS:
Enzyme-linked immunosorbent assay was used to detect serum immunoglobulin (Ig) G antibodies against a synthetic 24-mer peptide corresponding to the second extracellular loop of human M(2) mAChR. The effects of both total serum IgG and affinity-purified antipeptide antibodies on the contractile activity and adenosine 3', 5'-cyclic monophosphate (cAMP) production in rat esophageal strips were also tested. RESULTS: Circulating IgG antibodies from chagasic achalasia patients recognized the M(2)-peptide more often than those from non-chagasic achalasia patients (P < 0.0005) and normal subjects (P < 0.0001). A strong association between the existence of circulating anti-M(2) mAChR antibodies and the presence of achalasia in chagasic patients was found (P < 0.01). Both the total IgG fraction and anti-M(2)-peptide antibodies increased the basal tone, reduced the relaxant effect of isoproterenol, and decreased cAMP accumulation in esophageal strips, displaying a muscarinic agonist-like activity on M(2) mAChR. CONCLUSIONS:
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Authors | J C Goin, L Sterin-Borda, C R Bilder, L M Varrica, G Iantorno, M C Ríos, E Borda |
Journal | Gastroenterology
(Gastroenterology)
Vol. 117
Issue 4
Pg. 798-805
(Oct 1999)
ISSN: 0016-5085 [Print] United States |
PMID | 10500061
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Receptors, Muscarinic
- Cyclic AMP
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Topics |
- Adult
- Aged
- Animals
- Autoantibodies
(blood, pharmacology)
- Chagas Disease
(blood, complications, immunology)
- Cyclic AMP
(metabolism)
- Esophageal Achalasia
(complications, physiopathology)
- Esophagus
(drug effects, physiopathology)
- Female
- Humans
- In Vitro Techniques
- Male
- Middle Aged
- Muscle Contraction
- Muscle, Smooth
(physiopathology)
- Rats
- Rats, Wistar
- Receptors, Muscarinic
(immunology)
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