Hyperosmotic induction of the mitogen-activated protein kinase phosphatase MKP-1 in H4IIE rat hepatoma cells.

Abstract	The action of hyperosmotic stress on the MAP kinase phosphatase MKP-1 mRNA expression was studied in H4IIE rat hepatoma cells. Hyperosmotic (405 mosmol/L) challenge of the cells led to a transient expression of MKP-1 mRNA, which was maximal after 6-8 h and disappeared completely after 24 h. Hyperosmotic MKP-1 mRNA induction was preceded by a transient activation of the MAP kinases Erk-1, Erk-2, and JNK-2, which were not prerequisite for MKP-1 mRNA accumulation. However, the hyperosmolarity-induced MKP-1 mRNA expression was sensitive to antioxidants and to inhibition of p38 by SB203580. A reduced sensitivity of Erk-1/Erk-2 to other stimuli was found after prolonged hyperosmotic exposure. The data are consistent with a hyperosmolarity-induced MKP-1 expression via reactive oxygen intermediates and p38, which may participate in the termination of MAP kinase activation and contribute to desensitization of the MAP kinases after prolonged hyperosmotic exposure of the cells.
Authors	F Schliess, S Heinrich, D Häussinger
Journal	Archives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 351 Issue 1 Pg. 35-40 (Mar 01 1998) ISSN: 0003-9861 [Print] United States
PMID	9500841 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antioxidants Cell Cycle Proteins Enzyme Inhibitors Imidazoles Immediate-Early Proteins Pyridines RNA, Messenger Saline Solution, Hypertonic Protein Kinases Calcium-Calmodulin-Dependent Protein Kinases JNK Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases p38 Mitogen-Activated Protein Kinases MAP Kinase Kinase 4 Mitogen-Activated Protein Kinase Kinases Phosphoprotein Phosphatases Protein Phosphatase 1 Dual Specificity Phosphatase 1 Dusp1 protein, rat Protein Tyrosine Phosphatases SB 203580
Topics	Animals Antioxidants (pharmacology) Calcium-Calmodulin-Dependent Protein Kinases (antagonists & inhibitors, metabolism) Cell Cycle Proteins Dual Specificity Phosphatase 1 Enzyme Activation Enzyme Induction Enzyme Inhibitors (pharmacology) Gene Expression Imidazoles (pharmacology) Immediate-Early Proteins (antagonists & inhibitors, biosynthesis, genetics) JNK Mitogen-Activated Protein Kinases Kinetics Liver Neoplasms, Experimental (enzymology) MAP Kinase Kinase 4 Mitogen-Activated Protein Kinase 1 (metabolism) Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Phosphoprotein Phosphatases Protein Kinases (metabolism) Protein Phosphatase 1 Protein Tyrosine Phosphatases (antagonists & inhibitors, biosynthesis, genetics) Pyridines (pharmacology) RNA, Messenger (metabolism) Rats Saline Solution, Hypertonic Signal Transduction Tumor Cells, Cultured p38 Mitogen-Activated Protein Kinases

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!