Reactivity of B72.3 with adenocarcinomas. An immunohistochemical study of 476 cases.

Abstract

BACKGROUND:

The monoclonal antibody B72.3 has been shown to react with several common types of adenocarcinoma. However, the distribution of B72.3 reactivity in adenocarcinomas has not been well-enough defined to determine whether B72.3 immunostaining would be helpful in the diagnosis of adenocarcinoma of unknown origin.

METHODS:

Formaldehyde fixed, paraffin-embedded tissue from 476 adenocarcinomas from a variety of primary sites were studied using B72.3 and an avidin-biotin complex immunohistochemical technique.

RESULTS:

B72.3 immunoreactivity was seen in 75-100% of the adenocarcinomas from the ovary, endocervix, endometrium, distal esophagus/stomach, colon, lung, breast, prostate, pancreas, and bile ducts. Positive reactions were unusual in thyroid carcinomas (7%) and renal cell carcinomas (11%). B72.3 reactivity was not seen in the 20 hepatocellular carcinomas or 4 adrenal cortical carcinomas studied.

CONCLUSIONS:

B72.3 reacts with a wide variety of adenocarcinomas. Immunostaining with B72.3 may be helpful in excluding hepatocellular carcinoma in certain clinical settings.

• Authors: T S Loy, M B Nashelsky
• Journal: Cancer (Cancer) Vol. 72 Issue 8 Pg. 2495-8 (Oct 15 1993) ISSN: 0008-543X [Print] United States
• PMID: 8402466 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Glycoproteins
• Immunoglobulin G
• tumor-associated antigen 72

Topics

• Adenocarcinoma (diagnosis, immunology)
• Animals
• Antibodies, Monoclonal (immunology)
• Antigen-Antibody Reactions
• Antigens, Neoplasm (analysis, immunology)
• Female
• Glycoproteins (analysis, immunology)
• Humans
• Immunoglobulin G (immunology)
• Immunohistochemistry
• Male
• Mice