Immunoelectrophoretic studies on human small intestinal brush border proteins. The residual isomaltase in sucrose intolerant patients.

Abstract

Small intestinal biopsies from three patients with sucrose intolerance (sucrase-isomaltase deficiency) were studied by means of immunoelectrophoresis and enzymatic assays. All patients lacked sucrase activity (less than 1 unit/g protein). One of the patients had a substantial isomaltase activity (7.8 unit/g protein). Immunoelectrophoresis revealed the presence of the isomaltase polypeptide of the sucrase-isomaltase in this patient. None of the biopsies showed any precipitate that might represent a modified inactive sucrase protein.

• Authors: H Skovbjerg, P A Krasilnikoff
• Journal: Pediatric research (Pediatr Res) Vol. 15 Issue 3 Pg. 214-8 (Mar 1981) ISSN: 0031-3998 [Print] United States
• PMID: 7220143 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Membrane Proteins
• Sucrose
• Glycoside Hydrolases
• Sucrase-Isomaltase Complex
• Oligo-1,6-Glucosidase
• Sucrase

Topics

• Adult
• Carbohydrate Metabolism, Inborn Errors (enzymology)
• Cell Membrane (enzymology)
• Child, Preschool
• Glycoside Hydrolases (metabolism)
• Humans
• Immunoelectrophoresis, Two-Dimensional
• Jejunum (enzymology, ultrastructure)
• Membrane Proteins (analysis)
• Microvilli (enzymology)
• Oligo-1,6-Glucosidase (metabolism)
• Sucrase (metabolism)
• Sucrase-Isomaltase Complex (metabolism)
• Sucrose (metabolism)