Abstract |
This study aimed to explore the efficacy of polysaccharides from bergamot (BP) in alleviating DSS-induced colitis. Results showed that BP was primarily composed of two components, BP-1 and BP-2, with similar monosaccharide compositions to BP (mainly glucose and xylose) and molecular weights (Mw) of 4.50 × 105 and 2.35 × 105 Da. This study found BP relieved disease symptoms such as weight loss and colon shortening in mice with colitis. Gut microbiota and metabolomics analysis revealed that the BP could also promote the proliferation of beneficial bacteria such as Bifidobacteria, Butyrivibrio, and Blautia, resulting in increased levels of SCFAs and L-phenylalanine, which were associated with phenylalanine, tyrosine, and tryptophan metabolism pathways. Further analysis validated the inflammatory activity of L-phenylalanine. Hence, BP may relieve colitis symptoms by regulating the gut microbiota and metabolism, which reduced inflammation and enhanced the expression of tight junctional proteins (TJ proteins) and mucin in the intestine.
|
Authors | Yiren Yang, Yuwei Zhang, Jiangping Song, Yaqian Li, Liuyang Zhou, Hongtao Xu, Kaizhang Wu, Jie Gao, Mouming Zhao, Yang Zheng |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 253
Issue Pt 7
Pg. 127335
(Dec 31 2023)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 37820919
(Publication Type: Journal Article)
|
Copyright | Copyright © 2023. Published by Elsevier B.V. |
Chemical References |
- Phenylalanine
- Dextran Sulfate
- Polysaccharides
|
Topics |
- Animals
- Mice
- Colitis, Ulcerative
(chemically induced, drug therapy)
- Gastrointestinal Microbiome
- Colitis
(chemically induced, drug therapy)
- Inflammation
- Colon
- Phenylalanine
- Dextran Sulfate
- Disease Models, Animal
- Mice, Inbred C57BL
- Polysaccharides
(pharmacology, therapeutic use)
|