The objective of this study was to compare the
protein profile of the acquired enamel pellicle (AEP) formed in vivo in patients with or without
gastroesophageal reflux disease (
GERD), and with or without erosive
tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1)
GERD and ETW; 2)
GERD without ETW; and 3) control (without
GERD). The AEP formed 120 min after prophylaxis was collected from the lingual/palatal surfaces. The samples were subjected to mass spectrometry (nLC-ESI-MS/MS) and label-free quantification by
Protein Lynx Global Service software. A total of 213
proteins were identified, or 119, 92 and 106 from each group, respectively. Group 2 showed a high number of phosphorylated and
calcium-binding proteins. Twenty-three
proteins were found in all the groups, including
14-3-3 protein zeta/delta and 1-
phosphatidylinositol. Several intracellular
proteins that join saliva after the exfoliation of oral mucosa cells might have the potential to bind
hydroxyapatite, or participate in forming supramolecular aggregates that bind to precursor
proteins in the AEP.
Proteins might play a central role in protecting the dental surface against
acid dissolution.