Abstract | BACKGROUND: Gut microbiota are involved in the onset and development of chronic intestinal inflammation. The recently described endocannabinoidome (eCBome), a diverse and complex system of bioactive lipid mediators, has been reported to play a role in various physio- pathological processes such as inflammation, immune responses and energy metabolism. The eCBome and the gut microbiome (miBIome) are closely linked and form the eCBome - miBIome axis, which may be of special relevance to colitis. METHODS: RESULTS: GF mice showed increased levels of anti-inflammatory eCBome lipids (LEA, OEA, DHEA and 13- HODE-EA) in the healthy state and higher MPO activity. DNBS elicited reduced inflammation in GF mice, having lower colon weight/length ratios and lower expression levels of Il1b, Il6, Tnfa and neutrophil markers compared to one or both of the other DNBS-treated groups. Il10 expression was also lower and the levels of several N-acyl ethanolamines and 13-HODE-EA levels were higher in DNBS-treated GF mice than in CR and ABX mice. The levels of these eCBome lipids negatively correlated with measures of colitis and inflammation. CONCLUSIONS: These results suggest that the depletion of the gut microbiota and subsequent differential development of the gut immune system in GF mice is followed by a compensatory effect on eCBome lipid mediators, which may explain, in part, the observed lower susceptibility of GF mice to develop DNBS-induced colitis.
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Authors | Tommaso Venneri, Giada Giorgini, Nadine Leblanc, Nicolas Flamand, Francesca Borrelli, Cristoforo Silvestri, Vincenzo Di Marzo |
Journal | Lipids in health and disease
(Lipids Health Dis)
Vol. 22
Issue 1
Pg. 63
(May 15 2023)
ISSN: 1476-511X [Electronic] England |
PMID | 37189092
(Publication Type: Journal Article)
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Copyright | © 2023. The Author(s). |
Chemical References |
- Dinitrobenzenes
- 2,4-dinitrofluorobenzene sulfonic acid
- Lipids
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Topics |
- Mice
- Animals
- Dinitrobenzenes
(adverse effects)
- Colitis
(chemically induced, genetics, metabolism)
- Inflammation
- Lipids
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