Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells.

Abstract

Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs' efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative.

• Authors: Abdullah Alsrhani, Abozer Y Elderdery, Badr Alzahrani, Nasser A N Alzerwi, Maryam Musleh Althobiti, Musaed Rayzah, Bandar Idrees, Ahmed M E Elkhalifa, Suresh K Subbiah, Pooi Ling Mok
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 28 Issue 7 (Apr 04 2023) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 37049991 (Publication Type: Journal Article)

Chemical References

• visnagin
• Antioxidants
• Proto-Oncogene Proteins c-akt
• Phosphatidylinositol 3-Kinases
• TOR Serine-Threonine Kinases

Topics

• Humans
• Female
• Triple Negative Breast Neoplasms (drug therapy)
• Breast Neoplasms (drug therapy, pathology)
• Apoptosis
• Antioxidants (therapeutic use)
• Proto-Oncogene Proteins c-akt
• Phosphatidylinositol 3-Kinases
• Cell Line, Tumor
• Nanoparticles
• TOR Serine-Threonine Kinases