APTX acts in DNA double-strand break repair in a manner distinct from XRCC4.

Abstract

Aprataxin (APTX), the product of the causative gene for hereditary neurogenerative syndromes Ataxia-oculomotor apraxia 1 and early onset ataxia with oculomotor apraxia and hypoalbuminemia, has an enzymatic activity of removing adenosine monophosphate from DNA 5'-end, which arises from abortive ligation by DNA ligases. It is also reported that APTX physically binds to XRCC1 and XRCC4, suggesting its involvement in DNA single-strand break repair (SSBR) and DNA double-strand break repair (DSBR) via non-homologous end joining pathway. Although the involvement of APTX in SSBR in association with XRCC1 has been established, the significance of APTX in DSBR and its interaction with XRCC4 have remained unclear. Here, we generated APTX knock-out (APTX-/-) cell from human osteosarcoma U2OS through CRISPR/Cas9-mediated genome editing system. APTX-/- cells exhibited increased sensitivity toward ionizing radiation (IR) and Camptothecin in association with retarded DSBR, as shown by increased number of retained γH2AX foci. However, the number of retained 53BP1 foci in APTX-/- cell was not discernibly different from wild-type cells, in stark contrast to XRCC4-depleted cells. The recruitment of GFP-tagged APTX (GFP-APTX) to the DNA damage sites was examined by laser micro-irradiation and live-cell imaging analysis using confocal microscope. The accumulation of GFP-APTX on the laser track was attenuated by siRNA-mediated depletion of XRCC1, but not XRCC4. Moreover, the deprivation of APTX and XRCC4 displayed additive inhibitory effects on DSBR after IR exposure and end joining of GFP reporter. These findings collectively suggest that APTX acts in DSBR in a manner distinct from XRCC4.

• Authors: Rikiya Imamura, Mizuki Saito, Mikio Shimada, Junya Kobayashi, Masamichi Ishiai, Yoshihisa Matsumoto
• Journal: Journal of radiation research (J Radiat Res) Vol. 64 Issue 3 Pg. 485-495 (May 25 2023) ISSN: 1349-9157 [Electronic] England
• PMID: 36940705 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

Chemical References

• APTX protein, human
• DNA
• DNA-Binding Proteins
• Nuclear Proteins
• X-ray Repair Cross Complementing Protein 1
• XRCC1 protein, human

Topics

• Humans
• Cerebellar Ataxia
• DNA (metabolism)
• DNA Breaks, Double-Stranded
• DNA Damage
• DNA End-Joining Repair
• DNA Repair
• DNA-Binding Proteins (metabolism)
• Nuclear Proteins (metabolism)
• X-ray Repair Cross Complementing Protein 1 (genetics, metabolism)