Hederagenin ameliorates cisplatin-induced acute kidney injury via inhibiting long non-coding RNA A330074k22Rik/Axin2/β-catenin signalling pathway.

Abstract

BACKGROUND:

Acute kidney injury (AKI), a kidney disease with high morbidity and mortality, is characterized by a dramatic decline in renal function. Hederagenin (HDG), a pentacyclic triterpenoid saponin isolated from astragalus membranaceus, has been shown to have significant anti-inflammatory effects on various diseases. However, the effects of HDG on renal injury and inflammation in AKI has not been elucidated.

METHODS:

In this research, mice model of AKI was established by intraperitoneal injection of cisplatin in vivo, the inflammatory model of renal tubular epithelial cells was established by LPS stimulation in vitro, and HDG was used to intervene in vitro and in vivo models. Transcriptome sequencing was used to analyze the alterations of LncRNA and mRNA expression in AKI model and LncRNA-A330074k22Rik (A33) knockdown cells, respectively. Renal in situ electrotransfer knockdown plasmid was used to establish mice model of AKI with low expression of A33 in kidney.

RESULTS:

The results showed that HDG effectively alleviate cisplatin-induced kidney injury and inflammation in mice. Transcriptome sequencing results showed that multiple LncRNAs in kidney of AKI model exhibited significant changes, among which LncRNA-A33 had the most obvious change trend. Subsequent results showed that A33 was highly expressed in kidney of AKI mice and LPS-induced renal tubular cells. After in situ renal electroporation knockdown plasmid down-regulated A33 in kidney of AKI mice, it was found that inhibition of A33 could significantly relieve cisplatin-induced kidney injury and inflammation of AKI, while HDG could effectively suppress the expression of A33 in vitro and in vivo, respectively. Subsequently, transcriptome sequencing was again used to analyze the changes in mRNA expression of renal tubular cells after A33 knockdown by siRNA. The results showed that a large number of inflammation-related signaling pathways were down-regulated, Axin2 and its downstream β-catenin signal were significantly inhibited. Cell recovery test showed that HDG inhibited Axin2/β-catenin signal by down-regulating A33, and improved kidney injury and inflammation of AKI.

CONCLUSION:

Taken together, HDG significantly ameliorated cisplatin-induced kidney injury through LncRNA-A330074k22Rik/Axin2/β-catenin signal axis, which providing a potential therapeutic approach for the treatment of AKI.

• Authors: Ke-Huan Xie, Xiao-Heng Liu, Jian Jia, Xia Zhong, Rang-Yue Han, Rui-Zhi Tan, Li Wang
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 112 Pg. 109247 (Nov 2022) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 36155281 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Cisplatin
• RNA, Long Noncoding
• beta Catenin
• hederagenin
• Lipopolysaccharides
• RNA, Small Interfering
• Oleanolic Acid
• Anti-Inflammatory Agents
• Saponins
• RNA, Messenger
• Axin2 protein, mouse

Topics

• Mice
• Animals
• Cisplatin (therapeutic use)
• RNA, Long Noncoding (genetics, metabolism)
• beta Catenin (metabolism)
• Lipopolysaccharides (pharmacology)
• RNA, Small Interfering (metabolism)
• Mice, Inbred C57BL
• Acute Kidney Injury (chemically induced, drug therapy, genetics)
• Oleanolic Acid (pharmacology)
• Kidney
• Inflammation (metabolism)
• Disease Models, Animal
• Anti-Inflammatory Agents (therapeutic use)
• Saponins (pharmacology)
• RNA, Messenger (metabolism)