Ginkgolide A Participates in LPS-Induced PMVEC Injury by Regulating miR-224 and Inhibiting p21 in a Targeted Manner.

Abstract

Most studies have focused on the protective effects of ginkgolide A against ischemia/reperfusion-induced cardiomyopathy and injury of the brain, liver, and other organs, but there are few reports about the protection of lung tissues. This study was designed to clarify the protection of ginkgolide A against lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cell (PMVEC) injury. PMVECs were extracted and fell into control, LPS, and ginkgolide A groups. Next, we delved into the growth activity and apoptosis rate of cells via the CCK-8 assay and Hoechst staining, independently. Beyond that, western blotting (WB) was implemented for measurement of the expressions of cyclin D1, cyclin-dependent kinase 4 (CDK4), and CDK inhibitor (p21) that pertained to the cell cycle. The target sites of ginkgolide A were confirmed by miRNA array and real-time quantitative PCR. The relationship between miR-224 and p21 was analyzed using dual-luciferase reporter gene assay. Compared with the control group, the LPS group and ginkgolide A group had significantly decreased cell growth activity and relative expressions of cyclin D1 and CDK4 and elevated apoptosis rate and p21 expression. Pronounced elevations were observable in the cell growth activity and expressions of cyclin D1, CDK4, and p21, while the ginkgolide A group presented with a reduced apoptosis rate in comparison with the LPS group (P < 0.05). MiR-224 was the target of ginkgolide A, which had targeted regulatory effects on p21. Ginkgolide A can modulate miR-224 expression and regulate p21 expression in a targeted manner to enhance the resistance of PMVECs to LPS-induced cell apoptosis.

• Authors: Zhonglin Liu, Yan Yang
• Journal: Contrast media & molecular imaging (Contrast Media Mol Imaging) Vol. 2022 Pg. 6384334 ( 2022) ISSN: 1555-4317 [Electronic] England
• PMID: 36134118 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Zhonglin Liu and Yan Yang.

Chemical References

• Cyclin-Dependent Kinase Inhibitor p21
• Ginkgolides
• Lactones
• Lipopolysaccharides
• MicroRNAs
• Cyclin D1
• Cyclin-Dependent Kinase 4
• ginkgolide A

Topics

• Apoptosis
• Cyclin D1
• Cyclin-Dependent Kinase 4 (metabolism, pharmacology)
• Cyclin-Dependent Kinase Inhibitor p21 (metabolism, pharmacology)
• Endothelial Cells (metabolism)
• Ginkgolides
• Lactones
• Lipopolysaccharides (pharmacology)
• MicroRNAs (genetics, pharmacology)