Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Smilax china L. is a well-known traditional medicinal plant. In China, it is a common anti- cancer drug that has been inherited for thousands of years. Some in vitro and in vivo studies have confirmed its potential lipid-lowering, anti-inflammatory and anti- ovarian cancer effects. However, there is no research on the material basis and mechanism of the rhizome of Smilax china L. against hepatocellular carcinoma. AIM OF THE STUDY: METHODS: The potential targets and active components of Smilax china L. against hepatocellular carcinoma were screened by transcriptomics, network pharmacology and molecular docking. Microscale Thermophoresis (MST) detection was used to verify the affinity of small molecule compounds with potential proteins and protein- protein interaction. The Extract from HepG2 cells was used to measure the expression of glycolysis-related proteins, glucose consumption and lactate production. The expression of apoptosis-related factors and glycolysis-related proteins in vivo was detected by immunohistochemistry. RESULTS: CONCLUSION:
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Authors | Chen Wang, Qin Zhou, Song-Tao Wu |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 296
Pg. 115469
(Oct 05 2022)
ISSN: 1872-7573 [Electronic] Ireland |
PMID | 35718053
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier B.V. All rights reserved. |
Chemical References |
- Coumarins
- Glucosides
- Plant Extracts
- scopolin
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Topics |
- Carcinoma, Hepatocellular
(drug therapy, metabolism)
- Coumarins
- Glucosides
- Glycolysis
- Humans
- Liver Neoplasms
(drug therapy, metabolism)
- Molecular Docking Simulation
- Network Pharmacology
- Plant Extracts
(pharmacology)
- Smilax
(chemistry)
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