Abstract | Objective: Methods: Results: A total of 1004 proteins were identified, of which 109 proteins were differentially expressed between the CGE and DC groups. These differentially expressed proteins were primarily involved in hepatic fibrosis/hepatic stellate cell activation and immune/ inflammation-related pathways. In the disease and biofunction analysis, these proteins were mainly associated with the adhesion of blood cells, leukocyte migration, cholesterol transport, and transport of lipids. Twelve candidate biomarkers were validated by PRM-based proteomics, and proteins involved in the cholesterol metabolism pathway were further verified. APOA1, APOC3, adiponectin and PCSK9 concentrations were increased in CGE patients compared with healthy controls (P=0.0123, 0.1136, 0.5760, and 0.0019, respectively). Conclusion: This report describes the first application of a TMT-PRM-ELISA workflow to identify and validate CGE-specific biomarkers in serum. APOA1 and PCSK9 have been confirmed to be increased in CGE patients, demonstrating that proteins involved in cholesterol metabolism are also implicated in the development of CGE. These findings contribute to pathogenesis research and biomarker discovery in CGE.
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Authors | Peng Liu, Jianqiang Wu, Dandan Sun, Haolong Li, Zhihong Qi, Xiaoyue Tang, Wei Su, Yongzhe Li, Xuzhen Qin |
Journal | Frontiers in immunology
(Front Immunol)
Vol. 13
Pg. 855513
( 2022)
ISSN: 1664-3224 [Electronic] Switzerland |
PMID | 35677050
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 Liu, Wu, Sun, Li, Qi, Tang, Su, Li and Qin. |
Chemical References |
- Adiponectin
- Apolipoproteins
- Biomarkers
- Proteome
- PCSK9 protein, human
- Proprotein Convertase 9
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Topics |
- Adiponectin
- Apolipoproteins
- Biomarkers
- Cryoglobulinemia
(metabolism)
- Humans
- Proprotein Convertase 9
(metabolism)
- Proteome
- Proteomics
(methods)
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