Abstract | Objectives: Methods: Human vascular endothelial cells (EA.hy926) were cultured and pretreated with reparixin at concentrations of 0-1 mg/ml. To simulate ischemia, the cells were exposed to simulated ischemia solution for 60 min. Then, the cells were given complete medium as reperfusion followed by treatment with reparixin and incubated for 24 h. Cell viability was tested using MTT assay. Results: Percentages of cell viability of reparixin-treated groups of 0.0625 mg/mL (67.88 ± 7.82% control) and 0.125 mg/mL (84.28 ± 4.68% control) were significantly higher than that of the IR group (44.31 ± 4.64% control) at P < 0.05. The percentage of cell viability in the 0.125 mg/mL reparixin-treated group was not significantly different compared to the control. Conclusion: Pretreatment and treatment of endothelial cells with reparixin, which is a CXCL-8 receptor inhibitor, demonstrated a protective effect on cell viability after simulated ischemia-reperfusion. However, further studies to investigate the underlying mechanisms are needed.
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Authors | Piyanuch Thitiwuthikiat, Tamonlak Ta-Uea, Theeraya Ponghan, Saranya Meebua, Duangduan Siriwittayawan, Teonchit Nuamchit |
Journal | International journal of health sciences
(Int J Health Sci (Qassim))
2022 May-Jun
Vol. 16
Issue 3
Pg. 20-24
ISSN: 1658-3639 [Print] Saudi Arabia |
PMID | 35599941
(Publication Type: Journal Article)
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Copyright | Copyright: © International Journal of Health Sciences. |