Peptidomic Analysis on Mouse Lung Tissue Reveals AGDP as a Potential Bioactive Peptide against Pseudorabies Virus Infection.

Abstract

Pseudorabies virus (PRV) infection could cause severe histopathological damage via releasing multiple factors, including cytokines, peptides, etc. Here, peptidomic results showed that 129 peptides were identified in PRV-infected mouse lungs and were highly involved in the process of PRV infection. The role of one down-regulated biological peptide (designated as AGDP) during PRV infection was investigated. To verify the expression profiles of AGDP in response to PRV infection, the expression level of the precursor protein of AGDP mRNA was significantly decreased in PRV-infected mouse lungs and cells. The synthesized AGDP-treating cells were less susceptible to PRV challenges than the controls, as demonstrated by the decreased virus production and gE expression. AGDP not only inhibited the expression of TNF-α and IL-8 but also appeared to suppress the extracellular release of high-mobility group box 1 (HMGB1) by inhibiting the output of nuclear HMGB1 in cells. AGDP could also inhibit the degradation of IκBα and the phosphorylation levels of P65 after PRV infection. In total, our results revealed many meaningful peptides involved in PRV infection, thereby enhancing the current understanding of the host response to PRV infection, and how AGDP may serve as a promising candidate for developing novel anti-PRV drugs.

• Authors: Yijie Ma, Shimao Tian, Qianhui Wan, Yingying Kong, Chang Liu, Ke Tian, Hongya Ning, Xiaodong Xu, Baomin Qi, Guihong Yang
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 6 (Mar 18 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 35328729 (Publication Type: Journal Article)

Chemical References

• Cytokines
• HMGB1 Protein

Topics

• Animals
• Cytokines
• HMGB1 Protein
• Herpesvirus 1, Suid
• Lung (pathology)
• Mice
• Pseudorabies (drug therapy)