Abstract | BACKGROUND: METHODS AND RESULTS: Crude methanolic extracts (CMEs) of 16 selected plants were prepared by using maceration method and subjected to phytochemical assays for identification of major phytometabolites particularly cancer chemopreventive antioxidant constituents. Total flavonoid content of all plants CME was > 0.6 mg/ml exhibiting the Cichorium intybus contains comparatively highest amount of total flavonoid content (0.53 mg/ml). Scanvenging activity of all plants was determined having IC50 ranges between 2 and 88 (µg/ml) while Moringa oleifera revealed the maximum scavenging activity (IC50 2.03 µg/ml). Comparative cytotoxicity of plant extracts was evaluated in HUH and MCF-7 cell lines using 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) colorimetric assay. The nine active plant extracts i.e. Fagonia cretica, Argemone Mexicana, Rubus fruticosus, M. oleifera, Punica granatum, Cichorium intybus, Xanthium strumarium, Carissa opaca, Cyperus rotundus were identified based on their high antiproliferative activity > 50% against cancer cell lines and subjected to relative expression studies. Modulation of TGFβ signaling molecules (i.e.TGFβR1, 2 & 3, SMAD3, SMAD5) and ubiquitous proteins i.e. SMURF1 and SMURF2 genetic expression by potent extracts was determined by RT-PCR using GAPDH (housekeeping gene) as gene of reference. CONCLUSIONS: This present study revealed that CME of Fagonia cretica and Argemone mexicana significantly inhibit TGF beta mediated signaling cascade by downregulating the gene expression fold change > 1 of TGFβR 1, 2 & 3 and receptor associated complex protein SMAD3 as compared to control.
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Authors | Ammara Jabeen, Qaisar Mansoor, Tayyaba Zainab, Ammad Ahmad Farooqi, Mazhar Qayyum, Aqsa Mansha, Arifa Shakeel, Muhammad Sheeraz Ahmad |
Journal | Molecular biology reports
(Mol Biol Rep)
Vol. 49
Issue 6
Pg. 4171-4178
(Jun 2022)
ISSN: 1573-4978 [Electronic] Netherlands |
PMID | 35301659
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s), under exclusive licence to Springer Nature B.V. |
Chemical References |
- Antioxidants
- Flavonoids
- Plant Extracts
- Receptors, Transforming Growth Factor beta
- Transforming Growth Factor beta
- SMURF1 protein, human
- SMURF2 protein, human
- Ubiquitin-Protein Ligases
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Topics |
- Antioxidants
(pharmacology)
- Flavonoids
(pharmacology)
- Humans
- MCF-7 Cells
- Neoplasms
- Plant Extracts
(chemistry, pharmacology)
- Receptors, Transforming Growth Factor beta
(genetics)
- Transforming Growth Factor beta
(genetics, metabolism)
- Ubiquitin-Protein Ligases
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