A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.

Abstract

Polypropylene (PP) mesh is well-known as a gold standard of all prosthetic materials of choice for the reinforcement of soft tissues in case of hernia, organ prolapse, and urinary incontinence. The adverse effects that follow surgical mesh implantation remain an unmet medical challenge. Herein, it is outlined a new approach to allow viability and adhesion of human menstrual blood-derived mesenchymal stromal cells (MenSCs) on PP surgical meshes. A multilayered fibrin coating, based on fibrinogen and thrombin from a commercial fibrin sealant, was optimized to guarantee a homogeneous and stratified film on PP mesh. MenSCs were seeded on the optimized fibrin-coated meshes and their adhesion, viability, phenotype, gene expression, and immunomodulatory capacity were fully evaluated. This coating guaranteed MenSC viability, adhesion and did not trigger any change in their stemness and inflammatory profile. Additionally, MenSCs seeded on fibrin-coated meshes significantly decreased CD4+ and CD8+ T cell proliferation, compared to in vitro stimulated lymphocytes (p < 0.0001). Hence, the proposed fibrin coating for PP surgical meshes may allow the local administration of stromal cells and the reduction of the exacerbated inflammatory response following mesh implantation surgery. Reproducible and easy to adapt to other cell types, this method undoubtedly requires a multidisciplinary and translational approach to be improved for future clinical uses.

• Authors: Federica Marinaro, Joana M Silva, Alexandre A Barros, Ivo M Aroso, Juan C Gómez-Blanco, Isaac Jardin, Jose J Lopez, María Pulido, María Ángeles de Pedro, Rui L Reis, Francisco Miguel Sánchez-Margallo, Javier G Casado, Esther López
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 24 (Dec 13 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 34948187 (Publication Type: Journal Article)

Chemical References

• Coated Materials, Biocompatible
• Fibrin Tissue Adhesive
• Polypropylenes
• Fibrin

Topics

• Adult
• Cell Adhesion (physiology)
• Cell Separation (methods)
• Coated Materials, Biocompatible (chemistry, pharmacology)
• Female
• Fibrin (metabolism)
• Fibrin Tissue Adhesive (pharmacology)
• Humans
• Materials Testing
• Menstruation (blood)
• Mesenchymal Stem Cells (cytology)
• Polypropylenes (blood, chemistry)
• Prostheses and Implants
• Surgical Mesh
• Tissue Adhesions (pathology)