Abstract | BACKGROUND:
Low-level light therapy ( LLLT) is widely used for the photobiomodulation of cell behavior. Recent studies have shown that LLLT affects the proliferation and migration of various types of mesenchymal stem cells (MSCs). However, there is a lack of studies investigating the effect of LLT on enhancing the immunomodulatory properties of tonsil-derived MSCs (T-MSCs). OBJECTIVE: METHODS: We isolated T-MSCs from human palatine tonsils and evaluated the ingredients of T-MSCs-CM. The effect of T-MSCs-CM treated with LLLT was evaluated in a mouse model of allergic rhinitis (AR). We randomly divided the mice into four groups (negative control, positive control, T-MSCs-CM alone, and T-MSCs-CM treated with LLLT). To elucidate the therapeutic effect, we assessed rhinitis symptoms, serum immunoglobulin (Ig), the number of inflammatory cells, and cytokine expression. RESULTS: We identified increased expression of immunomodulatory factors, such as HGF, TGF-β, and PGE, in T-MSCs-CM treated with LLLT, compared to T-MSCs-CM without LLLT. Our animal study demonstrated reduced allergic symptoms and lower expression of total IgE and OVA-specific IgE in the LLLT-treated T-MSCs-CM group compared to the AR group and T-MSCs-CM alone. Moreover, we found that T-MSCs-CM treated with LLLT showed significantly decreased infiltration of eosinophils, neutrophils, and IL-17 cells in the nasal mucosa and reduced IL-4, IL-17, and IFN-γ expression in OVA-incubated splenocytes compared to the AR group. CONCLUSIONS:
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Authors | In-Su Park, Dong-Kyu Kim, Ji Hye Kim, Jun-Sang Bae, Eun Hee Kim, Shin Hyuk Yoo, Young-Jun Chung, Lele Lyu, Ji-Hun Mo |
Journal | American journal of rhinology & allergy
(Am J Rhinol Allergy)
Vol. 36
Issue 2
Pg. 261-268
(Mar 2022)
ISSN: 1945-8932 [Electronic] United States |
PMID | 34738483
(Publication Type: Journal Article)
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Chemical References |
- Anti-Allergic Agents
- Cytokines
- Ovalbumin
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Topics |
- Animals
- Anti-Allergic Agents
(therapeutic use)
- Cytokines
(metabolism)
- Disease Models, Animal
- Mesenchymal Stem Cells
(metabolism)
- Mice
- Mice, Inbred BALB C
- Nasal Mucosa
(metabolism)
- Ovalbumin
- Palatine Tonsil
- Rhinitis, Allergic
(drug therapy)
- Secretome
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