Abstract |
DPP-IV plays a key role for regulation of glucose metabolism in the body. The object of this study was to obtain DPP-IV inhibitors from discarded but protein-rich Penaeus vannamei (P. vannamei) head, and to explore the potential mechanism between DPP-IV and its inhibitors. P. vannamei head protein was hydrolyzed by five food grade proteases, respectively. The animal protease hydrolysate showed the highest inhibitory active. Then the hydrolysate was sequentially separated by ultrafiltration, gel filtration chromatography and reversed phase high-performance liquid chromatography (RP-HPLC), the peptides sequences were identified by LC-MS/MS and four potential peptides YPGE, VPW, HPLY, YATP showed superior DPP-IV inhibitory activity. Meanwhile, molecular docking effectively explored their mechanism through formed hydrogen bonds and hydrophobic regions. The four peptides showed better DPP-IV inhibitory activity stability with heating treatment, pH (1-10) treatment, and in vitro gastrointestinal digestion. Our results demonstrated that the protein hydrolysate from discarded P. vannamei head can be considered as a promising natural source of DPP-IV inhibitor for helping to improve glycaemic control in Type 2 diabetes.
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Authors | Xi Xiang, Meng Lang, Yan Li, Xia Zhao, Huimin Sun, Weiwei Jiang, Ling Ni, Yishan Song |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 1186
Pg. 122990
(Dec 01 2021)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 34735973
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Arthropod Proteins
- Dipeptidyl-Peptidase IV Inhibitors
- Peptides
- Protein Hydrolysates
- Dipeptidyl Peptidase 4
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Topics |
- Animals
- Arthropod Proteins
(chemistry)
- Chromatography, High Pressure Liquid
- Dipeptidyl Peptidase 4
(chemistry)
- Dipeptidyl-Peptidase IV Inhibitors
(chemistry, isolation & purification)
- Mass Spectrometry
- Molecular Docking Simulation
- Penaeidae
(chemistry)
- Peptides
(chemistry, isolation & purification)
- Protein Hydrolysates
(chemistry)
- Ultrafiltration
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