Bombesin Peptide Conjugated Water-Soluble Chitosan Gallate-A New Nanopharmaceutical Architecture for the Rapid One-Pot Synthesis of Prostate Tumor Targeted Gold Nanoparticles.

Abstract

PURPOSE:

We report herein bombesin peptide conjugated water-soluble chitosan gallate as a template for rapid one-pot synthesis of gold nanoparticles (AuNPs) with capabilities to target receptors on prostate cancer cells.

METHODS:

Water-soluble chitosan (WCS), anchored with gallic acid (GA) and LyslLys3 (1,4,7,10-tetraazacyclo dodecane-1,4,7,10-tetraacetic acid) bombesin 1-14 (DBBN) peptide, provides a tumor targeting nanomedicine agent. WCS nanoplatforms provide attractive strategies with built-in capabilities to reduce gold (III) to gold nanoparticles with stabilizing and tumor-targeting capabilities. WCS-GA-DBBN encapsulation around gold nanoparticles affords optimum in vitro stability.

RESULTS:

The DBBN content in the WCS-GA-DBBN sample was ~27%w/w. The antioxidant activities of WCS-GA and WCS-GA-DBBN nanocolloids were enhanced by 12 times as compared to the nascent WCS. AuNPs with a desirable hydrodynamic diameter range of 40-60 nm have been efficiently synthesized using WCS-GA and WCS-GA-DBBN platforms. The AuNPs were stable over 4 days after preparation and ~3 days after subjecting to all relevant biological fluids. The AuNPs capped with WCS-GA-DBBN peptide exhibited superior cellular internalization into prostate tumor (PC-3) cells with evidence of receptor mediated endocytosis.

CONCLUSION:

The AuNPs capped with WCS-GA-DBBN exhibited selective affinity toward prostate cancer cells. AuNPs conjugated with WCS-GA-DBBN serve as a new generation of theranostic agents for treating various neoplastic diseases, thus opening-up new applications in oncology.

• Authors: Theeranan Tangthong, Thananchai Piroonpan, Velaphi C Thipe, Menka Khoobchandani, Kavita Katti, Kattesh V Katti, Wanvimol Pasanphan
• Journal: International journal of nanomedicine (Int J Nanomedicine) Vol. 16 Pg. 6957-6981 ( 2021) ISSN: 1178-2013 [Electronic] New Zealand
• PMID: 34675516 (Publication Type: Journal Article)
• Copyright: © 2021 Tangthong et al.

Chemical References

• Peptides
• chitosan gallate
• Water
• Gold
• Chitosan
• Bombesin

Topics

• Bombesin
• Cell Line, Tumor
• Chitosan (analogs & derivatives)
• Gold
• Humans
• Male
• Metal Nanoparticles
• Peptides
• Prostatic Neoplasms (drug therapy)
• Water