Cross-Sectional Associations between HDL Structure or Function, Cell Membrane Fatty Acid Composition, and Inflammation in Elderly Adults.

Abstract

BACKGROUND:

Cell membrane fatty acid composition has been related to inflammation and cardiovascular disease (CVD) risk. Dysregulation of HDL function is also considered a CVD risk factor.

OBJECTIVES:

We aimed to investigate whether the content of cell membrane fatty acids and HDL functionality are linked to each other as well as to inflammation.

METHODS:

This cross-sectional analysis involved 259 participants (mean age: 67.9 y) with overweight/obesity (mean BMI: 29.5 kg/m2) from a coronary artery disease case-control study nested within the PREDIMED (PREvención con DIeta MEDiterránea) trial for which HDL functional parameters [apoA-I, apoA-IV, and apoC-III; cholesterol efflux capacity (CEC); HDL oxidative inflammatory index (HOII); sphingosine-1-phosphate (S1P); serum amyloid A (SAA); and complement-3 (C3) protein] were quantified. We also assessed 22 fatty acids in blood cell membranes using GC and inflammatory markers (IFN-γ and IL-1b, IL-6, IL-8, and IL-10) in serum. Associations of HDL-related variables with cell membrane fatty acids and with inflammatory markers were assessed using multivariable linear regression analyses with elastic net penalty.

RESULTS:

ApoA-I, apoC-III, CEC, HOII, S1P, and SAA, but not apoA-IV and C3 protein, were associated with membrane fatty acids. S1P and SAA were directly associated with IL-6, whereas apoA-I and C3 protein showed inverse associations with IL-6. Specific fatty acids including myristic acid (14:0) and long-chain n-6 fatty acids being negatively and positively associated with IL-8, respectively, were also found to be positively associated with SAA.

CONCLUSIONS:

This study suggests interrelations between indicators of inflammation and both blood cell membrane fatty acid composition and HDL structure/functional parameters in a Mediterranean population at high CVD risk.This trial was registered at www.isrctn.com as ISRCTN35739639.

• Authors: Jananee Muralidharan, Christopher Papandreou, Maria T Soria-Florido, Aleix Sala-Vila, Gemma Blanchart, Ramon Estruch, Miguel A Martínez-González, Dolores Corella, Emilio Ros, Miguel Ruiz-Canela, Montse Fito, Jordi Salas-Salvadó, Mònica Bulló
• Journal: The Journal of nutrition (J Nutr) Vol. 152 Issue 3 Pg. 789-795 (03 03 2022) ISSN: 1541-6100 [Electronic] United States
• PMID: 34637509 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of the American Society for Nutrition.

Chemical References

• Apolipoprotein A-I
• Apolipoprotein C-III
• Biomarkers
• Cholesterol, HDL
• Fatty Acids
• Interleukin-6
• Interleukin-8

Topics

• Aged
• Apolipoprotein A-I
• Apolipoprotein C-III
• Biomarkers
• Cardiovascular Diseases (epidemiology)
• Case-Control Studies
• Cell Membrane
• Cholesterol, HDL
• Cross-Sectional Studies
• Fatty Acids
• Humans
• Inflammation
• Interleukin-6
• Interleukin-8