Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma.

Abstract

IFIX, a newly discovered member of the interferon-inducible HIN-200 family, has been identified as a tumor suppressor in breast cancer; however, the involvement of IFIX in oral cancer are poorly understood. Here, we demonstrate a relationship between the level of IFIX expression and the invasive or migratory abilities of oral squamous cell carcinoma. Higher IFIX expression significantly correlated with clinicopathological parameters such as the histopathological grade of clinical samples. In vitro, IFIX overexpression suppressed the invasiveness of human tongue squamous cell carcinoma CAL-27 cells, and this inhibitory effect was mediated by stabilization of the cytoskeleton through various cytokeratins along with downregulation of paxillin, an intracellular adaptor protein that promotes tumor invasion. This inhibitory effect does not appear to affect the transformation of cancer stem-like cells in this cell culture model. Altogether, these data provide novel insights into the tumor-suppressive function of IFIX, namely, stabilization of the cancer cell cytoskeleton.

• Authors: Shan Wang, Fang Li, Haixia Fan
• Journal: Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 19593 (10 01 2021) ISSN: 2045-2322 [Electronic] England
• PMID: 34599264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021. The Author(s).

Chemical References

• Antineoplastic Agents
• Nuclear Proteins
• PYHIN1 protein, human
• Paxillin

Topics

• Antineoplastic Agents (pharmacology)
• Carcinoma, Squamous Cell (pathology)
• Cell Line
• Cell Line, Tumor
• Cell Movement
• Cells, Cultured
• Cytoskeleton (metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Mouth Neoplasms (pathology)
• Neoplasm Invasiveness
• Nuclear Proteins (genetics, metabolism, pharmacology)
• Paxillin (metabolism)
• Tongue Neoplasms (pathology)