Anti-damage effect of theaflavin-3'-gallate from black tea on UVB-irradiated HaCaT cells by photoprotection and maintaining cell homeostasis.

Abstract

Keratinocytes are rich in lipids and are the main sensitive cells to ultraviolet (UV) rays. Theaflavins are the core functional components of black tea and are known as the "soft gold" in tea. In this study, ultraviolet-B (UVB) irradiation caused apoptosis and necrosis of human epidermal keratinocytes (HaCaT). EGCG and the four theaflavins had anti-UVB damage activity, among which theaflavin-3'-gallate (TF3'G) had the best activity. The results of biophysical and molecular biology experiments showed that TF3'G has anti-damage effects on UVB-irradiated HaCaT cells through the dual effects of photoprotection and maintenance of cell homeostasis. That is, TF3'G preincubation could absorb UV rays, reduce the accumulation of aging-related heterochromatin (SAHF) formation, increase mitochondrial membrane potential, downregulate NF-κB inflammation pathways, inhibit the formation of cytotoxic aggregates, and protect biological macromolecules Structure, etc. The accumulation of conjugated π bonds and the balance benzoquinone are the core functional structure of TF3'G with high efficiency and low toxicity. The study indicates that TF3'G has the potential to inhibit the photoaging and intrinsic aging of skin cells.

• Authors: Xin Zheng, Meiyan Feng, Juan Wan, Yulan Shi, Xinya Xie, Wenjing Pan, Baozhu Hu, Yingzi Wang, Haitao Wen, Kunbo Wang, Shuxian Cai
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 224 Pg. 112304 (Nov 2021) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 34536907 (Publication Type: Journal Article)
• Copyright: Copyright © 2021. Published by Elsevier B.V.

Chemical References

• Antioxidants
• Biflavonoids
• Radiation-Protective Agents
• Tea
• theaflavine gallate
• Gallic Acid
• Catechin
• epigallocatechin gallate

Topics

• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Biflavonoids (isolation & purification, pharmacology)
• Catechin (analogs & derivatives, isolation & purification, pharmacology)
• Cellular Senescence (drug effects)
• Gallic Acid (analogs & derivatives, isolation & purification, pharmacology)
• HaCaT Cells
• Homeostasis (drug effects)
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Radiation-Protective Agents (pharmacology)
• Spectrophotometry, Ultraviolet
• Spectroscopy, Fourier Transform Infrared
• Tea (chemistry)
• Ultraviolet Rays