Abstract | BACKGROUND: METHODS: LPS was intranasally injected into wild-type and transgenic mice. Gain and loss of VEGF-C/VEGFR-3 signalling function experiments employed adenovirus-mediated intranasal delivery of VEGF-C (Ad- VEGF-C vector) and soluble VEGFR-3 (sVEGFR-3) or anti-VEGFR-3 blocking antibodies and mice with a deletion of VEGFR-3 in myeloid cells. RESULTS: The early phase of lung injury was significantly alleviated by the overexpression of VEGF-C with increased levels of bronchoalveolar lavage (BAL) fluid interleukin-10 (IL-10), but worsened in the later phase by VEGFR-3 inhibition upon administration of Ad-sVEGFR-3 vector. Injection of anti-VEGFR-3 antibodies to mice in the resolution phase inhibited recovery from lung injury. The VEGFR-3-deleted mice had a shorter survival time than littermates and more severe lung injury in the resolution phase. Alveolar macrophages in the resolution phase digested most of the extrinsic apoptotic neutrophils and VEGF-C/VEGFR-3 signalling increased efferocytosis via upregulation of integrin αv in the macrophages. We also found that incubation with BAL fluid from acute respiratory distress syndrome (ARDS) patients, but not from controls, decreased VEGFR-3 expression and the efficiency of IL-10 expression and efferocytosis in human monocyte-derived macrophages. CONCLUSIONS:
VEGF-C/VEGFR-3 signalling in macrophages ameliorates experimental lung injury. This mechanism may also provide an explanation for ARDS resolution.
|
Authors | Masahiro Yamashita, Miyuki Niisato, Yasushi Kawasaki, Sinem Karaman, Marius R Robciuc, Yuji Shibata, Yoji Ishida, Ryosuke Nishio, Tomoyuki Masuda, Tamotsu Sugai, Masao Ono, Rubin M Tuder, Kari Alitalo, Kohei Yamauchi |
Journal | The European respiratory journal
(Eur Respir J)
Vol. 59
Issue 4
(04 2022)
ISSN: 1399-3003 [Electronic] England |
PMID | 34446463
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright ©The authors 2022. For reproduction rights and permissions contact [email protected]. |
Chemical References |
- Lipopolysaccharides
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factor C
- Interleukin-10
- Vascular Endothelial Growth Factor Receptor-3
|
Topics |
- Acute Lung Injury
(metabolism)
- Animals
- Humans
- Interleukin-10
(adverse effects, metabolism)
- Lipopolysaccharides
- Macrophages, Alveolar
(metabolism)
- Mice
- Mice, Inbred C57BL
- Respiratory Distress Syndrome
- Vascular Endothelial Growth Factor A
(metabolism)
- Vascular Endothelial Growth Factor C
(metabolism)
- Vascular Endothelial Growth Factor Receptor-3
(metabolism)
|