Potential effect of EGCG on the anti-tumor efficacy of metformin in melanoma cells.

Abstract

Metformin, a first-line drug for type 2 diabetes mellitus, has been recognized as a potential anti-tumor agent in recent years. Epigallocatechin-3-gallate (EGCG), as the dominant catechin in green tea, is another promising adjuvant agent for tumor prevention. In the present work, the potential effect of EGCG on the anti-tumor efficacy of metformin in a mouse melanoma cell line (B16F10) was investigated. Results indicated that EGCG and metformin exhibited a synergistic effect on cell viability, migration, and proliferation, as well as signal transducer and activator of transcription 3/nuclear factor-κB (STAT3/NF-κB) pathway signaling and the production of inflammation cytokines. Meanwhile, the combination showed an antagonistic effect on cell apoptosis and oxidative stress levels. The combination of EGCG and metformin also differentially affected the nucleus (synergism) and cytoplasm (antagonism) of B16F10 cells. Our findings provide new insight into the potential effects of EGCG on the anti-tumor efficacy of metformin in melanoma cells.

• Authors: An'an Xu, Jeehyun Lee, Yueling Zhao, Yuefei Wang, Xiaoli Li, Ping Xu
• Journal: Journal of Zhejiang University. Science. B (J Zhejiang Univ Sci B) Vol. 22 Issue 7 Pg. 548-562 (Jul 15 2021) ISSN: 1862-1783 [Electronic] China
• PMID: 34269008 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Cytokines
• NF-kappa B p50 Subunit
• NFKB1 protein, human
• STAT3 Transcription Factor
• STAT3 protein, human
• Catechin
• Metformin
• epigallocatechin gallate

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis
• Catechin (administration & dosage, analogs & derivatives)
• Cell Line, Tumor
• Cell Movement
• Cell Nucleus (metabolism)
• Cell Proliferation
• Cell Survival
• Cytokines (metabolism)
• Cytoplasm (metabolism)
• Inflammation
• Melanoma (drug therapy)
• Melanoma, Experimental
• Metformin (administration & dosage)
• Mice
• NF-kappa B p50 Subunit (metabolism)
• Oxidative Stress
• Phosphorylation
• STAT3 Transcription Factor (metabolism)
• Skin Neoplasms (drug therapy)
• Spectrum Analysis, Raman