Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves (ESL) were reported to have neuroprotective function and are also used to treat cranial and cerebral
traumas as a
traditional Chinese medicine and food herbage plant. However, there has been no previous study on ESL treatment for
stroke at the level of
lipid disorders. To clarify the mechanism of ESL in treating
ischemic stroke, this study was carried out from 3 aspects, namely, the regulation of
lipid disorders, protection of the nervous system, as well as anti-inflammatory and
antioxidant actions. This study established a lipidomics research strategy that was developed by UPLC-Q-TOF/MS analysis. The quantification of
neurotransmitters in the serum and brain tissue of rats was performed using UPLC-TQ/MS. Also, we quantified the oxidative stress and inflammatory reaction by measuring the contents of SOD, MDA, TNF-α,
IL-6, and
IL-10 via the ELISA kits for serum and brain tissue. According to UPLC-Q-TOF/MS-based lipidomics analysis, 27 lipidomics
biomarkers were identified in this study, including PC, PE, SM, and TG, which were distributed in various
lipid metabolic pathways, including
glycerophospholipid,
linoleic acid,
alpha-linolenic acid, glycerolipid,
sphingolipid, and
arachidonic acid metabolism pathways. By reversing the changes in the
lipid content caused by the disease, ESL has a
therapeutic effect on
ischemic stroke. Furthermore, quantitative results of
neurotransmitters indicated that they can be regulated by ESL. Finally, the results of ELISA showed that ESL can treat
ischemic stroke to a certain extent by reducing the oxidative and inflammatory damage. Therefore, ESL may play a therapeutic role in the treatment of
ischemic stroke in different ways. This research preliminarily revealed the mechanism of ESL in the treatment of
ischemic stroke and provided support for the further application of ESL.