Abstract | BACKGROUND: PURPOSE: METHODS: RESULTS: The OLE + SPE combination significantly ameliorated atopic dermatitis symptoms, including dermatitis scores, and reduced epidermal thickness and infiltration of different inflammatory cells in mice with DNCB-induced atopic dermatitis. It also significantly reduced the number of CD4+, CD8+, and CD4+/CD69+ T cells; immunoglobulin E-producing B cells (CD23+/B220+) in the axillary lymph nodes; CD3+ T-cell eosinophils ( chemokine- chemokine receptor 3+/CD11b+) in the skin; and CD3+ T cells, immunoglobulin E-producing B cells (CD23+/B220+), and eosinophils in peripheral blood mononuclear cells. Additionally, the experimental combination lowered levels of serum immunoglobulin E and histamine, as well as Th2-mediated cytokines, and interleukin-4, -5, and -13, whereas it increased the levels of Th1-mediated cytokine interferon-γ in splenocytes. Furthermore, the preparation significantly restored expression of the skin barrier-related proteins filaggrin, sirtuin 1, and claudin 1, and also reduced the expression of the inflammatory cytokine interleukin-6 and chemokine- chemokine receptor 3, as well as the pruritus-related cytokine interleukin-31 and interleukin-31 receptor, in atopic dermatitis skin lesions. CONCLUSION: Taken together, our findings indicate that administration of a combination of OLE and SPE can alleviate atopic dermatitis symptoms by regulating immune balance and skin barrier function and may be an effective therapeutic option for the treatment of atopic dermatitis.
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Authors | Young-Sil Lee, Hyung Won Ryu, Won-Kyung Yang, Mi Hyeon Park, Yang-Chun Park, Doo-Young Kim, Hyuk Joon Kwon, Soo-Young Kim, Sei-Ryang Oh, Seung-Hyung Kim |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 82
Pg. 153407
(Feb 2021)
ISSN: 1618-095X [Electronic] Germany |
PMID | 33571899
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier GmbH. All rights reserved. |
Chemical References |
- 2,4-dinitrobenzene
- Cytokines
- Dinitrobenzenes
- FLG protein, human
- Filaggrin Proteins
- Intermediate Filament Proteins
- Plant Extracts
- Immunoglobulin E
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Topics |
- Animals
- Cytokines
(metabolism)
- Dermatitis, Atopic
(drug therapy)
- Dinitrobenzenes
(chemistry, toxicity)
- Disease Models, Animal
- Filaggrin Proteins
- Immunoglobulin E
(blood)
- Intermediate Filament Proteins
(metabolism)
- Leukocytes, Mononuclear
(metabolism)
- Male
- Mice
- Olea
(chemistry)
- Plant Extracts
(pharmacology, therapeutic use)
- Skin
(drug effects, metabolism)
- Th2 Cells
(drug effects)
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