Abstract |
Guaiane-type sesquiterpene lactones are naturally occurring compounds which have attracted attention due to their array of biological activities. In this study, chlorinated guaianolides 1-8, isolated from plants of the genus Centaurea, were evaluated against the human leukemia cell lines HL-60, U-937, a specific U-937 cell line that overexpresses the anti-apoptotic Bcl-2 protein and the human melanoma cell line SK-MEL-1. This established the relevant structure-growth inhibition relationships. Chlorohyssopifolins A (1), C (3) and D (4) and linichlorin A (6) were the most potent compounds in terms of inducing growth inhibition in the four cell lines. IC50 values were below 10 μM in all cases. Chlorohyssopifolins A (1) and D (4) and linichlorin A (6) were potent apoptotic inducers in human U-937 leukemia cells, as determined by fluorescent microscopy and flow cytometry, and their mechanism of action was associated with cytochrome c release, caspase activation and poly(ADP-ribose)polymerase cleavage. Overall this study shows that guaianolides induce cytotoxicity against human tumor cells and provides important insights into the cell death pathways that are involved.
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Authors | Francisco Estévez-Sarmiento, Ester Saavedra, Mercedes Ruiz-Estévez, Francisco León, José Quintana, Ignacio Brouard, Francisco Estévez |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 21
Issue 24
(Dec 21 2020)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 33371413
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- Cytotoxins
- Lactones
- Sesquiterpenes, Guaiane
- Cytochromes c
- Poly(ADP-ribose) Polymerases
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
- Cytochromes c
(metabolism)
- Cytotoxins
(pharmacology)
- Humans
- Lactones
(pharmacology)
- Leukemia
(drug therapy, pathology)
- Poly(ADP-ribose) Polymerases
(metabolism)
- Sesquiterpenes, Guaiane
(chemistry)
- U937 Cells
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