Intra-articular injection of anti-inflammatory peptide-loaded glycol chitosan/fucoidan nanogels to inhibit inflammation and attenuate osteoarthritis progression.

Abstract

Glycol chitosan/fucoidan nanogels loaded with anti-inflammatory peptide KAFAK (GC/Fu@KAFAK NGs) were fabricated based on the electrostatic interaction and genipin cross-linking methods. The prepared NGs had an average size of 286.3 ± 5.0 nm and positive surface charge of 14.0 ± 0.2 mV. The anti-inflammatory and chondro-protective effects of GC/Fu@KAFAK NGs were evaluated on interlecukin-1β (IL-1β)-stimulated rat chondrocytes. We found that GC/Fu@KAFAK NGs not only inhibited the expression of inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), but also enhanced the expression of chondrogenic markers type II collagen, aggrecan, and Sox9. More importantly, in rat osteoarthritis (OA) model, the intra-articular (IA) injection of GC/Fu@KAFAK NGs reduced glycosaminoglycan loss and diminished inflammatory cytokine release. In addition, GC/Fu@KAFAK NGs showed good biocompatibility both in vitro and in vivo. In conclusion, IA inject-able GC/Fu@KAFAK NGs might have great potential in OA treatment.

• Authors: Tao Li, Junjun Yang, Changmei Weng, Pingju Liu, Yang Huang, Shuo Meng, Rong Li, Liu Yang, Cheng Chen, Xiaoyuan Gong
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 170 Pg. 469-478 (Feb 15 2021) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 33359610 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Collagen Type II
• Cytokines
• Interleukin-1beta
• Interleukin-6
• Nanogels
• Polysaccharides
• Tumor Necrosis Factor-alpha
• glycol-chitosan
• Chitosan
• fucoidan

Topics

• Animals
• Anti-Inflammatory Agents (administration & dosage)
• Cells, Cultured
• Chitosan (chemistry)
• Chondrocytes (drug effects, metabolism)
• Collagen Type II (metabolism)
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation (drug therapy, metabolism)
• Injections, Intra-Articular (methods)
• Interleukin-1beta (metabolism)
• Interleukin-6 (metabolism)
• Male
• Nanogels (administration & dosage)
• Osteoarthritis (drug therapy, metabolism)
• Polysaccharides (chemistry)
• Rats
• Rats, Sprague-Dawley
• Tumor Necrosis Factor-alpha (metabolism)