Abstract | BACKGROUND: METHODS: Transwell migration and invasion assays were performed to detect the migration and invasion of BC cells. Western blot assay was carried out to measure epithelial-mesenchymal transition (EMT)-related proteins in BC cells, including E-cadherin, N-cadherin, and fibronectin. Quantitative real-time polymerase chain reaction was conducted to determine the enrichment of miR-139-5p and ADP-ribosylation factor 6 (ARF6) in BC tissues and cells. The protein expression of ARF6 in BC tissues and cells was measured by western blot assay. The target of miR-139-5p was predicted by starBase software, and the target relationship between miR-139-5p and ARF6 in BC cells was confirmed by dual- luciferase reporter assay. RESULTS: SEV suppressed the migration, invasion, and EMT of BC cells, especially in the high-concentration SEV group. The level of miR-139-5p was lower in BC tissues and cells than that in paired normal tissues and normal mammary epithelial cells MCF-10A. MiR-139-5p was upregulated in BC cells treated with SEV. ARF6 was upregulated in BC tissues and cells compared with that in corresponding normal tissues and normal mammary epithelial cells MCF-10A. SEV reduced the mRNA and protein expression of ARF6 in BC cells. The accumulation of ARF6 or the interference of miR-139-5p reversed the suppressive effects of SEV treatment on the migration, invasion, and EMT of BC cells. MiR-139-5p bound to ARF6 and inversely modulated the level of ARF6 in BC cells. The transfection of si-ARF6 attenuated the promoting effects of miR-139-5p depletion on the migration, invasion, and EMT of BC cells treated with SEV. CONCLUSIONS: SEV suppressed the migration, invasion, and EMT of BC cells through downregulating the abundance of ARF6 by upregulating miR-139-5p. The miR-139-5p/ARF6 axis might be a promising target for the treatment of BC.
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Authors | Tongle Wu, Luwei Sun, Chuantao Wang, Peng Yu, Long Cheng, Yongmin Chen |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 258
Pg. 314-323
(02 2021)
ISSN: 1095-8673 [Electronic] United States |
PMID | 33317757
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- ADP-Ribosylation Factor 6
- Anesthetics, Inhalation
- MIRN139 microRNA, human
- MicroRNAs
- Sevoflurane
- ADP-Ribosylation Factors
- ARF6 protein, human
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Topics |
- ADP-Ribosylation Factor 6
- ADP-Ribosylation Factors
(metabolism)
- Anesthetics, Inhalation
(pharmacology)
- Cell Movement
(drug effects)
- Drug Evaluation, Preclinical
- Epithelial-Mesenchymal Transition
(drug effects)
- Gene Expression Regulation, Neoplastic
(drug effects)
- Humans
- MCF-7 Cells
- MicroRNAs
(metabolism)
- Sevoflurane
(pharmacology)
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