Pernicious
placenta previa with
placenta percreta (PP) is a catastrophic condition during pregnancy. However, the underlying pathogenesis remains unclear. In the present study, the placental tissues of normal cases and PP tissues of pernicious
placenta previa cases were collected to determine the expression profile of
protein-coding genes,
miRNAs, and lncRNAs through sequencing. Weighted gene co-expression network analysis (WGCNA), accompanied by
miRNA target prediction and correlation analysis, were employed to select potential
hub protein-coding genes and lncRNAs. The expression levels of selected
protein-coding genes, Wnt5A and MAPK13, were determined by quantitative PCR and immunohistochemical staining, and
lncRNA PTCHD1-AS and PAPPA-AS1 expression levels were determined by quantitative PCR and fluorescence in situ hybridization. The results indicated that 790
protein-coding genes, 382
miRNAs, and 541 lncRNAs were dysregulated in PP tissues, compared with normal tissues. WGCNA identified coding genes in the module (ME) black and ME turquoise modules that may be involved in the pathogenesis of PP. The selected potential
hub protein-coding genes, Wnt5A and MAPK13, were down-regulated in PP tissues, and their expression levels were positively correlated with the expression levels of PTCHD1-AS and PAPPA-AS1. Further analysis demonstrated that PTCHD1-AS and PAPPA-AS1 regulated Wnt5A and MAPK13 expression by interacting with specific
miRNAs. Collectively, our results provided multi-omics data to better understand the pathogenesis of PP and help identify predictive
biomarkers and therapeutic targets for PP.