In-vivo imaging revealed antigen-directed gingival B10 infiltration in experimental periodontitis.

Abstract

Our previous study demonstrated that IL-10 secreting B (B10) cells alleviate inflammation and bone loss in experimental periodontitis. The purpose of this study is to determine whether antigen-specificity is required for the local infiltration of B10 cells. Experimental periodontitis was induced in the recipient mice by placement of silk ligature with or without the presence of live Porphyromonas gingivalis (P. gingivalis). Donor mice were pre-immunized by intraperitoneal (IP) injection of formalin-fixed P. gingivalis, or PBS as non-immunized control. Spleen B cells were purified and treated with LPS and CpG for 48 h to expand the B10 population in vitro. Fluorescence-labelled B10 cells were transferred into the recipient mice by tail vein injection and were tracked on day 0, 3, 5 and 10 using IVIS Spectrum in vivo imaging system. The number of B10 cells and P. gingivalis-binding B cells were significantly increased after in vitro treatment of LPS and CpG. On day 5, the fluorescence intensity in gingival tissues was the highest in mice transferred with B10 cells from pre-immunized donor mice. Gingival expression of IL-6, TNF-α, RANKL/OPG ratio and periodontal bone loss in recipient mice were significantly reduced, and the expression of IL-10 and the number of CD19+ B cells were significantly increased after pre-immunized B10 cell transfer in the presence of antigen, compared to those with non-immunized B10 cell transfer or no antigen presence. This study suggests that antigen specificity dictate the local infiltration of B10 cells into periodontal tissue and these antigen-specific B10 cells promote anti-inflammatory responses.

• Authors: Yufeng Wang, Yang Hu, Keqing Pan, Hao Li, Shu Shang, Yuhua Wang, Guoyao Tang, Xiaozhe Han
• Journal: Biochimica et biophysica acta. Molecular basis of disease (Biochim Biophys Acta Mol Basis Dis) Vol. 1867 Issue 1 Pg. 165991 (01 01 2021) ISSN: 1879-260X [Electronic] Netherlands
• PMID: 33080346 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Antigens, Bacterial
• Cytokines

Topics

• Animals
• Antigens, Bacterial (immunology)
• B-Lymphocytes, Regulatory (immunology)
• Bacteroidaceae Infections (diagnostic imaging, immunology, microbiology)
• Cytokines (immunology)
• Diagnostic Imaging
• Gingiva (diagnostic imaging, immunology, microbiology)
• Mice
• Periodontitis (diagnostic imaging, immunology, microbiology)
• Porphyromonas gingivalis (immunology)