HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Retinal pigment epithelial cells secrete miR-202-5p-containing exosomes to protect against proliferative diabetic retinopathy.

Abstract
Previous studies have reported that endothelial-to-mesenchymal transition (EndoMT) contributes to pathological fibrosis in proliferative diabetic retinopathy (PDR). The hypothesis of our study was that exosomes from high glucose (HG)-treated ARPE19 cells reprogram endothelial cell behavior in HG conditions by transferring their genetic contents. Our study showed that ARPE19-derived exosomes were internalized by human umbilical vein endothelial cells (HUVECs). Additionally, miR-202-5p, a miRNA known to target TGFβR2, was enriched in ARPE19-derived exosomes. A dual luciferase reporter assay, qPCR, and western blotting were used to characterize the expression of miR-202-5p and phosphorylation of the TGF/Smad pathway proteins. We showed that miR-202-5p-containing exosomes suppressed HUVEC cell growth, migration, and tube formation. Furthermore, TGFβR2 was confirmed as the target of miR-202-5p. A dual luciferase reporter assay showed that TGFβR2 expression was negatively regulated by miR-202-5p. We also showed that miR-202-5p-containing exosomes suppressed HG-induced EndoMT. These collective results suggested that ARPE-derived exosomes may serve as significant mediators of cell-to-cell crosstalk to suppress EndoMT by transferring miR-202-5p through the TGF/Smad pathway, and may be a potential treatment for PDR patients.
AuthorsShun Gu, Yixiao Liu, Jian Zou, Wenjuan Wang, Tingting Wei, Xiaolu Wang, Lingpeng Zhu, Mengyuan Zhang, Jing Zhu, Tianhua Xie, Yong Yao, Liying Qiu
JournalExperimental eye research (Exp Eye Res) Vol. 201 Pg. 108271 (12 2020) ISSN: 1096-0007 [Electronic] England
PMID33007305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2020 Elsevier Ltd. All rights reserved.
Chemical References
  • MIRN202 microRNA, human
  • MicroRNAs
  • RNA
Topics
  • Apoptosis
  • Blotting, Western
  • Cells, Cultured
  • Diabetic Retinopathy (genetics, metabolism, pathology)
  • Exosomes (genetics, metabolism, ultrastructure)
  • Gene Expression Regulation
  • Humans
  • MicroRNAs (biosynthesis, genetics)
  • Microscopy, Electron, Transmission
  • RNA (genetics)
  • Retinal Pigment Epithelium (metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: