Abstract | BACKGROUND:
Preterm birth implies an array of respiratory diseases including apnea of prematurity and bronchopulmonary dysplasia (BPD). Caffeine has been introduced to treat apneas but also appears to reduce rates of BPD. Oxygen is essential when treating preterm infants with respiratory problems but high oxygen exposure aggravates BPD. This experimental study is aimed at investigating the action of caffeine on inflammatory response and cell death in pulmonary tissue in a hyperoxia-based model of BPD in the newborn rat. Material/Methods. Lung injury was induced by hyperoxic exposure with 80% oxygen for three (P3) or five (P5) postnatal days with or without recovery in ambient air until postnatal day 15 (P15). Newborn Wistar rats were treated with PBS or caffeine (10 mg/kg) every two days beginning at the day of birth. The effects of caffeine on hyperoxic-induced pulmonary inflammatory response were examined at P3 and P5 immediately after oxygen exposure or after recovery in ambient air (P15) by immunohistological staining and analysis of lung homogenates by ELISA and qPCR. RESULTS: CONCLUSIONS: The present study investigating the impact of caffeine on the inflammatory response, pulmonary cell degeneration and modulation of adenosine receptor expression, provides further evidence that caffeine acts as an antioxidative and anti-inflammatory drug for experimental oxygen-mediated lung injury. Experimental studies may broaden the understanding of therapeutic use of caffeine in modulating detrimental mechanisms involved in BPD development.
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Authors | Stefanie Endesfelder, Evelyn Strauß, Ivo Bendix, Thomas Schmitz, Christoph Bührer |
Journal | Oxidative medicine and cellular longevity
(Oxid Med Cell Longev)
Vol. 2020
Pg. 3840124
( 2020)
ISSN: 1942-0994 [Electronic] United States |
PMID | 32831996
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Stefanie Endesfelder et al. |
Chemical References |
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Topics |
- Animals
- Animals, Newborn
- Caffeine
(adverse effects)
- Disease Models, Animal
- Female
- Humans
- Lung Injury
(physiopathology)
- Oxygen
(adverse effects)
- Rats
- Rats, Wistar
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