Ameliorative effects and molecular mechanisms of vine tea on western diet-induced NAFLD.

Abstract

Non-alcoholic fatty liver disease (NAFLD) is a disease that is prevalent worldwide, and its prevention by dietary administration has recently been considered as an important strategy. In this study, we administered mice with vine tea polyphenol (VTP) extracted from Ampelopsis grossedentata, a Chinese herb, to investigate the preventive effect on western diet (WD)-induced NAFLD. Male C57BL/6N mice were fed either a normal diet (ND) or WD with or without VTP for 12 weeks. The results revealed that VTP supplementation decreased the serum levels of cholesterol and triglycerides, and reduced the accumulation of hepatic lipid droplets caused by WD. Molecular data revealed that VTP enhanced fatty acid oxidation by reactivating the WD-suppressed phosphorylation of AMP-activated protein kinaseα (AMPKα) and the expressions of peroxisome proliferator-activated receptor alpha (PPARα), carnitine palmitoyl transferase IA (CPT1A) and cytochrome P450, family 4, subfamily a1 (CYP4A1). VTP inhibited hepatic lipogenesis by reducing the WD-enhanced level of mature sterol regulatory element-binding protein 1 (SREBP1) and fatty acid synthase (FAS). Moreover, VTP activated nuclear factor (erythroid-derived 2)-like 2 (Nrf2)-mediated expressions of hemeoxygenase-1 (HO-1) and quinone oxidoreductase (NQO1), and reduced hepatic TBARS levels to prevent hepatic oxidative stress. On the other hand, VTP also increased intestinal zonula occludens-1 (ZO-1) expression and the relative abundance of gut Akkermansia, and reduced the ratio of Firmicutes/Bacteroidetes. Thus, VTP might prevent WD-induced NAFLD by balancing fatty acid oxidation and lipogenesis, hepatic oxidative stress, and gut microbiome, at least. These results suggest that vine tea, containing a high content of the bioactive compound dihydromyricetin, is a potential food resource for preventing NAFLD.

• Authors: Kun Xie , Xi He , Keyu Chen , Kozue Sakao , De-Xing Hou
• Journal: Food & function (Food Funct) Vol. 11 Issue 7 Pg. 5976-5991 (Jul 01 2020) ISSN: 2042-650X [Electronic] England
• PMID: 32666969 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Drugs, Chinese Herbal
• Flavonols
• Intracellular Signaling Peptides and Proteins
• Keap1 protein, mouse
• Kelch-Like ECH-Associated Protein 1
• Membrane Proteins
• NF-E2-Related Factor 2
• Nfe2l2 protein, mouse
• PPAR alpha
• SREBP cleavage-activating protein
• Teas, Herbal
• Cytochrome P-450 CYP4A
• CPT1B protein, mouse
• Carnitine O-Palmitoyltransferase
• AMPK alpha1 subunit, mouse
• AMP-Activated Protein Kinases
• dihydromyricetin

Topics

• AMP-Activated Protein Kinases (metabolism)
• Ampelopsis (chemistry)
• Animals
• Antioxidants (pharmacology, therapeutic use)
• Carnitine O-Palmitoyltransferase (metabolism)
• Cytochrome P-450 CYP4A (metabolism)
• Diet, High-Fat (adverse effects)
• Diet, Western (adverse effects)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Flavonols (pharmacology, therapeutic use)
• Gastrointestinal Microbiome (drug effects)
• Hep G2 Cells
• Humans
• Intracellular Signaling Peptides and Proteins (metabolism)
• Kelch-Like ECH-Associated Protein 1
• Lipid Metabolism (drug effects)
• Liver (drug effects, metabolism, pathology)
• Membrane Proteins (metabolism)
• Mice, Inbred C57BL
• NF-E2-Related Factor 2 (metabolism)
• Non-alcoholic Fatty Liver Disease (etiology, metabolism, prevention & control)
• Oxidative Stress (drug effects)
• PPAR alpha (metabolism)
• Phytotherapy
• Teas, Herbal