Ameliorative Effect of Ananas comosus on Cobalt Chloride-Induced Hypoxia in Caco2 cells via HIF-1α, GLUT 1, VEGF, ANG and FGF.

Abstract

In the present study, protective effects of Ananas comosus i.e., pineapple pulp (PA) against cobalt chloride (CoCl2)‑induced hypoxia in Caco-2 cells were evaluated. PA reduces levels of lipid peroxidation, reactive oxygen and nitrogen species. It was proved to be cytoprotective and increased anti-oxidant activity against CoCl2-induced hypoxia. The inference drawn from this experiment was CoCl2-induced hypoxia that regulates hypoxia-inducible factor-1 (HIF-1), a transcription factor. It was also confirmed that PA pre-treatment inhibited the expression of HIF-1α, thereby downregulating the hypoxia-associated gene/protein expressions such as GLUT-1, VEGF, ANG and FGF. Finally, supplementation of PA could help in snow-balling the digestive hormones like leptin and CCK in hypoxic conditions. Therefore, this report provides substantial proof of reducing the hypoxia-induced loss of appetite at high-altitude environments. Graphical Abstract.

• Authors: Anusha Maliyur Basavaraju, Naveen Shivanna, Chandrasekhar Yadavalli, Phani Kumar Garlapati, Anilakumar Kandangath Raghavan
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 199 Issue 4 Pg. 1345-1355 (Apr 2021) ISSN: 1559-0720 [Electronic] United States
• PMID: 32654099 (Publication Type: Journal Article)

Chemical References

• Glucose Transporter Type 1
• Hypoxia-Inducible Factor 1, alpha Subunit
• Vascular Endothelial Growth Factor A
• Cobalt
• Fibroblast Growth Factors
• cobaltous chloride

Topics

• Ananas (metabolism)
• Caco-2 Cells
• Cell Hypoxia
• Cobalt (toxicity)
• Fibroblast Growth Factors
• Glucose Transporter Type 1
• Humans
• Hypoxia
• Hypoxia-Inducible Factor 1, alpha Subunit
• Vascular Endothelial Growth Factor A (metabolism)