Roles of Endogenous IL-10 and IL-10-Competent and CD5+ B Cells in Autoimmune Thyroiditis in NOD.H-2h4 Mice.

Abstract

Interleukin (IL)-10 is a highly important anti-inflammatory cytokine in the immune system. CD1dhi and CD5+ B cells are both traditionally defined IL-10-secreting B cells. In recent years, a B cell group with combined markers of CD1dhi and CD5+ has been widely studied as it has been reported to suppress autoimmunity in mouse models of autoimmune diseases through IL-10 mechanisms. From the perspective of origination, CD1dhi and CD5+ B cells are developed from different B cell lineages. Whether the regulatory capacity of these 2 B cell groups is consistent with their ability to secrete IL-10 has not been determined. In this study, we generated IL-10 knockout NOD.H-2h4 mice to investigate the function of endogenous IL-10 in autoimmune thyroiditis and conducted adoptive transfer experiments to explore the respective roles of CD5+ and CD1dhi B cells. In our results, the IL-10-/- NOD.H-2h4 mice developed thyroiditis, similar to wild-type NOD.H-2h4 mice. The CD5+ B cells were more capable of secreting IL-10 than CD1dhi B cells in flow cytometric analysis, but the CD1dhi B cells showed more suppressive effects on thyroiditis development and autoantibody production, as well as Th17 cell response. In conclusion, endogenous IL-10 does not play an important role in autoimmune thyroiditis. CD1dhi B cells may play regulatory roles through mechanisms other than secreting IL-10.

• Authors: Jing Qin, Na Zhao, Shuo Wang, Shanshan Liu, Yongping Liu, Xuejiao Cui, Shiwei Wang, Yang Xiang, Chenling Fan, Yushu Li, Zhongyan Shan, Weiping Teng
• Journal: Endocrinology (Endocrinology) Vol. 161 Issue 4 (04 01 2020) ISSN: 1945-7170 [Electronic] United States
• PMID: 32152630 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © Endocrine Society 2020. All rights reserved. For permissions, please e-mail: [email protected].

Chemical References

• Autoantibodies
• Interleukin-10
• Thyroglobulin

Topics

• Animals
• Autoantibodies
• B-Lymphocytes (metabolism)
• Interleukin-10 (genetics, metabolism)
• Mice
• Mice, Inbred NOD
• Mice, Knockout
• Thyroglobulin (immunology)
• Thyroid Gland (metabolism)
• Thyroiditis, Autoimmune (metabolism)