Anti-Atopic Effect of Acorn Shell Extract on Atopic Dermatitis-Like Lesions in Mice and Its Active Phytochemicals.

Abstract

To investigate the potential effects of acorn shells on atopic dermatitis (AD), we utilized oxazolone (OX)- or 2,4-dinitrochlorobenzene (DNCB)-induced AD-like lesion mouse models. Our research demonstrates that Acorn shell extract (ASE) improved the progression of AD-like lesions, including swelling, which were induced by oxazolone on Balb/c mouse ears. Additionally, ASE significantly decreased the ear thickness (OX: 0.42 ± 0.01 mm, OX-ASE: 0.32 ± 0.02 mm) and epidermal thickness (OX: 75.3 ± 32.6 µm, OX-ASE: 46.1 ± 13.4 µm). The continuous DNCB-induced AD mouse model in SKH-1 hairless mice demonstrated that ASE improved AD-like symptoms, including the recovery of skin barrier dysfunction, Immunoglobulin E hyperproduction (DNCB: 340.1 ± 66.8 ng/mL, DNCB-ASE: 234.8 ± 32.9 ng/mL) and an increase in epidermal thickness (DNCB: 96.4 ± 21.9 µm, DNCB-ASE: 52.4 ± 16.3 µm). In addition, we found that ASE suppressed the levels of AD-involved cytokines, such as Tumor Necrosis Factor α, IL-1β, IL-25 and IL-33 in both animal models. Furthermore, gallic acid and ellagic acid isolated from ASE suppressed β-hexosaminidase release and IL-4 expression in RBL-2H3 cells. The acorn shell and its active phytochemicals have potential as a new remedy for the improvement of atopic dermatitis and other inflammatory diseases.

• Authors: Sullim Lee, Hyun Jegal, Sim-Kyu Bong, Kyeong-No Yoon, No-June Park, Myoung-Sook Shin, Min Hye Yang, Yong Kee Kim, Su-Nam Kim
• Journal: Biomolecules (Biomolecules) Vol. 10 Issue 1 (12 29 2019) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 31905797 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Dinitrochlorobenzene
• Phytochemicals
• Plant Extracts
• Oxazolone

Topics

• Animals
• Anti-Inflammatory Agents (chemistry, isolation & purification, pharmacology)
• Cell Line, Tumor
• Cytokines (antagonists & inhibitors, biosynthesis)
• Dermatitis, Atopic (drug therapy, metabolism, pathology)
• Dinitrochlorobenzene (chemistry, pharmacology)
• Disease Models, Animal
• Female
• Mice
• Mice, Hairless
• Mice, Inbred BALB C
• Oxazolone (chemistry, pharmacology)
• Phytochemicals (chemistry, isolation & purification, pharmacology)
• Plant Extracts (chemistry, isolation & purification, pharmacology)
• Quercus (chemistry)
• Rats