Abstract | PURPOSE: Recently, it has been demonstrated that Raman spectroscopy is able to differentiate between healthy parathyroid tissues and parathyroid adenoma based on the basis of a specific molecular fingerprint. However, to our knowledge, no previous studies have been performed to evaluate the metabolic profile of parathyroid adenoma. Therefore, we designed a proof of concept study aimed to investigate the glucose/ fatty acid metabolisms, in addition to the mitochondrial changes, in solitary parathyroid adenoma and in healthy parathyroid glands. METHODS: Nine females with primary hyperparathyroidism due to a solitary parathyroid adenoma and formal surgical indication for parathyroidectomy have been enrolled. At the time of surgery, the removed specimens were immediately submitted unfixed and a tissue slice of about 0.5 cm in diameter was obtained from the nodular lesion. The expression of selected metabolic enzymes and proteins has been evaluated by western blot analysis, using human parathyroid whole tissue lysates as control. RESULTS: CONCLUSIONS: Our findings highlight for the first time the parathyroid adenoma metabolic hallmarks that could represent potential molecular targets usable for the development of new pharmacological treatments, allowing to reduce surgical parathyroidectomy.
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Authors | Alessandra di Masi, Loris Leboffe, Armida Sodo, Gaia Tabacco, Roberto Cesareo, Marco Sbroscia, Isabella Giovannoni, Chiara Taffon, Pierfilippo Crucitti, Filippo Longo, Silvia Manfrini, Maria Antonietta Ricci, Paolo Ascenzi, Anna Crescenzi, Andrea Palermo |
Journal | Endocrine
(Endocrine)
Vol. 67
Issue 3
Pg. 699-707
(03 2020)
ISSN: 1559-0100 [Electronic] United States |
PMID | 31786773
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adenoma
(surgery)
- Female
- Humans
- Metabolome
- Parathyroid Glands
- Parathyroid Hormone
- Parathyroid Neoplasms
(surgery)
- Parathyroidectomy
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