Abstract | BACKGROUND: OBJECTIVES: In this study, we evaluated the role of eIF3b in cell proliferation and apoptosis in CML. MATERIAL AND METHODS: Samples from patients with CML and CML cell lines were used. Quantitative RT-PCR, siRNA transfection, flow cytometry, and western blot analysis were performed. RESULTS: Quantitative RT-PCR revealed that the expression of eIF3b mRNA in CML patients was higher than that in the non-malignant controls. The proliferation of CML cells decreased after transfection of the cells with siRNA. The proportion of cells in the G1 and S phases in the experimental group decreased after transfection, while the number of cells in the G2/M phase increased, as compared with the control group. The total cell apoptosis percentage in the sheIF3b group (transduction with lentivirus-anti-eIF3b in K562 cells) was higher than the shCtrl group (transduction with empty-vector lentivirus in K562 cells) after transfection. Caspase 3/7 activity was higher and the expression of anti-apoptotic protein BCL-2 was lower in the sheIF3b group than in the shCtrl group after transfection. CONCLUSIONS: Our results suggest that downregulation of eIF3b expression inhibits proliferation and induces apoptosis in CML cells.
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Authors | Laiquan Huang, Kun He, Jianxin Wang, Jiawei Yan, Yizhi Jiang, Zhongling Wei, Jun Zhang, Guangxi Li, Lili Sheng |
Journal | Advances in clinical and experimental medicine : official organ Wroclaw Medical University
(Adv Clin Exp Med)
Vol. 28
Issue 12
Pg. 1639-1645
(12 2019)
ISSN: 1899-5276 [Print] Poland |
PMID | 31778600
(Publication Type: Journal Article)
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Chemical References |
- Eukaryotic Initiation Factor-3
- RNA, Small Interfering
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Topics |
- Apoptosis
- Cell Proliferation
- Eukaryotic Initiation Factor-3
(genetics, metabolism)
- Humans
- K562 Cells
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
(genetics, metabolism, pathology)
- RNA Interference
- RNA, Small Interfering
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Cells, Cultured
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